ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLVHIISFL LFFQLSAAKA PPSKTSLINT HERRSIYSCY VGLRKETWGF NGSAICRYEP 

        70         80         90        100        110        120 
AIQSMLYCLY EDTHEKGYSN KTLEKGFEEM RQFCYTPKFL NMTDAEFYTS LDNGTYYIQD 

       130        140        150        160        170        180 
QPKAGINITY PIRLNTTLRK AYYDAYYGYY YNHDIPYYFG GIICAYFVGV MLLAGLIRFL 

       190        200        210        220        230        240 
NYTPIKKIMF QQKLVNYVRG YTTLPTLYEK HAEPFSYLKV ITGYLPTRFE TLVILGYLIL 

       250        260        270        280        290        300 
HTIFMAYKYQ YDPYHIIFAA HRAEVAHFVA YRSGILSFAH LPLIVLFAGR NNFLQLISGL 

       310        320        330        340        350        360 
KHTSFIVFHK WLGRMMFLDA IIHAAGFTNY YLYYKKWNTV RLRVYWKFGI ATTCLAGMLI 

       370        380        390        400        410        420 
FFSIAAFRRH YYETFMALHI VFAALFLYTC WEHVTNFSGI EWIYAAIAIW GVDRIVRITR 

       430        440        450        460        470        480 
IALLGFPKAD LQLVGSDLVR VTVKKPKKFW KAKPGQYVFV SFLRPLCFWQ SHPFTVMDSC 

       490        500        510        520        530        540 
VNDRELVIVL KAKKGVTKLV RNFVERKGGK ASMRLAIEGP YGSKSTAHRF DNVLLLAGGS 

       550        560        570        580        590        600 
GLPGPISHAL ELGKTTAASG KNFVQLVIAV RGLDMLNACK KELMALKGLN VQVHIYNSKQ 

       610        620        630        640        650        660 
ELASAEKISS NEVKNGETTA EKAPSSLSNS EKAPSESENT ELPLSLNDTS ISDLEFATFH 

       670        680        690        700        710 
VGRPNVEEIL NESVNHSGSL AVVCCGPPIF VDTARNQTAK AVIRNPSRMI EYLEEYQAW 

References and documentation are available.

Number of amino acids: 719

Molecular weight: 82015.34

Theoretical pI: 9.26

Amino acid composition: 
Ala (A)  58	  8.1%
Arg (R)  33	  4.6%
Asn (N)  32	  4.5%
Asp (D)  18	  2.5%
Cys (C)  12	  1.7%
Gln (Q)  17	  2.4%
Glu (E)  37	  5.1%
Gly (G)  44	  6.1%
His (H)  22	  3.1%
Ile (I)  50	  7.0%
Leu (L)  75	 10.4%
Lys (K)  45	  6.3%
Met (M)  16	  2.2%
Phe (F)  44	  6.1%
Pro (P)  28	  3.9%
Ser (S)  44	  6.1%
Thr (T)  42	  5.8%
Trp (W)  10	  1.4%
Tyr (Y)  47	  6.5%
Val (V)  45	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      3792
Hydrogen    H	      5804
Nitrogen    N	       966
Oxygen      O	      1012
Sulfur      S	        28

Formula: C3792H5804N966O1012S28
Total number of atoms: 11602

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   125780
Abs 0.1% (=1 g/l)   1.534, assuming all pairs of Cys residues form cystines


Ext. coefficient   125030
Abs 0.1% (=1 g/l)   1.524, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.27
This classifies the protein as stable.



Aliphatic index: 94.02

Grand average of hydropathicity (GRAVY): 0.044