ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLVRTTSLN VSRMPVPCLA RGIGILKGKY RLANLMNAQP SVRHVSSEIQ QKDQQAGESN 

        70         80         90        100        110        120 
TATDTGVIHK SDEETLIYFD NVYARTTSVW NPTLWYNLLL RNQSRDAVRE KIRNLASPPN 

       130        140        150        160        170        180 
NPIYGLELKS TIPVKRDGGV FATFVVPPKY TKAQVNSLIQ QNTARESSKN LLSYFTRASA 

       190        200        210        220        230        240 
FPVKGSPWIE DLRRLPSTTI VIKFQGPALT EEEIYSLFRR YGTIIDIFPP TAANNNVAKV 

       250        260        270        280        290        300 
RYRSFRGAIS AKNCVSGIEI HNTVLHIQYE NIRRGHLVSN FFTNHTRIAI PVLFALLSIF 

       310        320        330        340        350        360 
AVLVFDPIRE FSIEQKITHK YSLSWDNKFW KQLKTLTSST MTSIKYYWGG PDDNHQRKHL 

       370        380        390        400        410        420 
WEERIEKVND LKMWLEENNN TFVVIRGPRG SGKHDLVMQH TLQNRANVLY LDCDKLIKSR 

       430        440        450        460        470        480 
TDPMFLKNAA SQLGYFPIFP WIDSVTGVLD LTVQGLTGQK TGLSETKESR FRNMLTTSLM 

       490        500        510        520        530        540 
SIRRIALKNY KAFVSTGDGT VNVKEEDYLQ QHPEAKPVIV IDRFEGKSEI NGFVYKELSD 

       550        560        570        580        590        600 
WAAMLVQMNI AHVIFLTETV ASNQRLSESL PNQVFKNLIL SDASKENSRN YVLSQLEDYL 

       610        620        630        640        650        660 
YYNKKSKGEN VKEPESEKET AENNDSDSEA DTSVKKAEVI LNEKELQEID ASLEPLGGRM 

       670        680        690        700        710        720 
LDLQAFVRRV KSGEEPSEAV DKMIEQASEQ ITQMFLSDKI DSNKSAQAWE LIELLSANPV 

       730        740        750        760        770        780 
IPFHEIVNKP LFKAAPETGI MELENNGLIT VSRDRGVLQE IRPAKPLYRA AFTYLINDPE 

       790        800        810        820        830        840 
LAKVLKTRYL LKVVGFETGR IKKWEEELKP LGKVPDQKLF KTRLDYLSGK INASNAVITK 

       850 
CEEEIKNLSK 

References and documentation are available.

Number of amino acids: 850

Molecular weight: 96688.64

Theoretical pI: 8.98

Amino acid composition: 
Ala (A)  51	  6.0%
Arg (R)  47	  5.5%
Asn (N)  55	  6.5%
Asp (D)  37	  4.4%
Cys (C)   4	  0.5%
Gln (Q)  33	  3.9%
Glu (E)  64	  7.5%
Gly (G)  39	  4.6%
His (H)  14	  1.6%
Ile (I)  57	  6.7%
Leu (L)  85	 10.0%
Lys (K)  65	  7.6%
Met (M)  15	  1.8%
Phe (F)  33	  3.9%
Pro (P)  38	  4.5%
Ser (S)  65	  7.6%
Thr (T)  51	  6.0%
Trp (W)  12	  1.4%
Tyr (Y)  27	  3.2%
Val (V)  58	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 101
Total number of positively charged residues (Arg + Lys): 112

Atomic composition:

Carbon      C	      4330
Hydrogen    H	      6890
Nitrogen    N	      1184
Oxygen      O	      1284
Sulfur      S	        19

Formula: C4330H6890N1184O1284S19
Total number of atoms: 13707

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   106480
Abs 0.1% (=1 g/l)   1.101, assuming all pairs of Cys residues form cystines


Ext. coefficient   106230
Abs 0.1% (=1 g/l)   1.099, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.49
This classifies the protein as unstable.



Aliphatic index: 90.94

Grand average of hydropathicity (GRAVY): -0.395