ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLMAKPDVPS SILPRSSSIL PNSIETLDEN ESIEAQVNNV QSLALSSPNR DRSDDDDNNN 

        70         80         90        100        110        120 
NHDSVSIPPP IYDGYSSSSS DESQSVPSPP INLDHDDDEC QIPIRNTSQA LDDIDDDIWG 

       130        140        150        160        170        180 
DDDLPETRRP WTPNVSPGFG SDDDDDNDDD NSKNEPRKSL FYGFRQEPEP VTGVGAGLWN 

       190        200        210        220        230        240 
LGNSCFLNSV FQCFTHTVPL IESLLSFRYE VPCHCGNEFF CVIRAIRYHI EAALRPERCP 

       250        260        270        280        290        300 
IAPYFFFDNL NYFSPDFQRY QQEDAHEFLQ AFLEKLEICG SDRTSFRGDI TSQDVFSGRL 

       310        320        330        340        350        360 
ISGLRCCNCD YVSETYEKSV GLSLEIEDVD TLGSALESFT RVEKLDEQLT CDNCNEKVSK 

       370        380        390        400        410        420 
EKQLLLDKLP LVATFHLKRF KNNGLYMEKI YKHVKIPLEI DLQPYMRNIQ ENEVSTKYHL 

       430        440        450        460        470        480 
YALVEHFGYS VAYGHYSSYV RSAPKIWHHF DDSKVTRIDE DMVLSQDSYI LFYAREGTRW 

       490        500        510        520        530        540 
FSSVYEEMQP LVEASLLNSS PKSVLDSSTN GECLSEISYE NGDKASKPCD SAGVCNQHVK 

       550        560        570        580        590        600 
TKEDFVSLSN DDVFLSAESS SGEESPMGEL LDPLDPDDSY SPCTEKESDS CLAIERATIR 

       610        620        630        640        650        660 
DDFFPLLLDQ NQESSTSSPK LQERTFEMQL LQMEETTKSQ EPWKQPLSSI SNIADSMEAE 

       670        680        690 
FVYGDLMKKP SPRARELLDQ AISTNGSPPK KLKTT 

References and documentation are available.

Number of amino acids: 695

Molecular weight: 78632.91

Theoretical pI: 4.45

Amino acid composition: 
Ala (A)  27	  3.9%
Arg (R)  30	  4.3%
Asn (N)  36	  5.2%
Asp (D)  65	  9.4%
Cys (C)  18	  2.6%
Gln (Q)  27	  3.9%
Glu (E)  58	  8.3%
Gly (G)  27	  3.9%
His (H)  14	  2.0%
Ile (I)  33	  4.7%
Leu (L)  64	  9.2%
Lys (K)  32	  4.6%
Met (M)  11	  1.6%
Phe (F)  31	  4.5%
Pro (P)  44	  6.3%
Ser (S)  85	 12.2%
Thr (T)  28	  4.0%
Trp (W)   6	  0.9%
Tyr (Y)  25	  3.6%
Val (V)  34	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 123
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      3438
Hydrogen    H	      5278
Nitrogen    N	       914
Oxygen      O	      1143
Sulfur      S	        29

Formula: C3438H5278N914O1143S29
Total number of atoms: 10802

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    71375
Abs 0.1% (=1 g/l)   0.908, assuming all pairs of Cys residues form cystines


Ext. coefficient    70250
Abs 0.1% (=1 g/l)   0.893, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.54
This classifies the protein as unstable.



Aliphatic index: 72.50

Grand average of hydropathicity (GRAVY): -0.614