ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD 

        70         80         90        100        110        120 
KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN GDLVEYNKYL EEVEDIIYKL 

       130        140        150        160        170        180 
DHGIDVAKTE EKLRTYEELN KQLIMNNLER SRTEIESFEQ RQKFEKEMKL KKRLLERQIE 

       190        200        210        220        230        240 
EEERMNKEWT KKEIVNRLST TTQDINETIE GVKNTVKLKK SSARRKLEEL NRVLKNNPYF 

       250        260        270        280        290        300 
NSNVNVQNSR LKDAVPFTPF NGDREAHPRF TLKGSVYNDP FIKDLEHRKE FIASGFNTNY 

       310        320 
AYERVLTEAF MGLGCVISEE L 

References and documentation are available.

Number of amino acids: 321

Molecular weight: 38128.41

Theoretical pI: 5.86

Amino acid composition: 
Ala (A)   8	  2.5%
Arg (R)  22	  6.9%
Asn (N)  25	  7.8%
Asp (D)  20	  6.2%
Cys (C)   8	  2.5%
Gln (Q)   8	  2.5%
Glu (E)  40	 12.5%
Gly (G)  10	  3.1%
His (H)   4	  1.2%
Ile (I)  19	  5.9%
Leu (L)  26	  8.1%
Lys (K)  34	 10.6%
Met (M)   7	  2.2%
Phe (F)  17	  5.3%
Pro (P)  10	  3.1%
Ser (S)  13	  4.0%
Thr (T)  16	  5.0%
Trp (W)   1	  0.3%
Tyr (Y)  12	  3.7%
Val (V)  21	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      1683
Hydrogen    H	      2671
Nitrogen    N	       463
Oxygen      O	       516
Sulfur      S	        15

Formula: C1683H2671N463O516S15
Total number of atoms: 5348

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23880
Abs 0.1% (=1 g/l)   0.626, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.613, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.68
This classifies the protein as unstable.



Aliphatic index: 76.14

Grand average of hydropathicity (GRAVY): -0.811