ProtParam
User-provided sequence:
10 20 30 40 50 60
MLPEKRLLTP DDMKLWEESP TRAHFTKFII DLAESVKGHE NSQYKEPISE SINSMMNLLS
70 80 90 100 110 120
QIKDITQKHP VIKDADSSRF GKVEFRDFYD EVSRNSRKIL RSEFPSLTDE QLEQLSIYLD
130 140 150 160 170 180
ESWGNKRRID YGSGHELNFM CLLYGLYSYG IFNLSNDSTN LVLKVFIEYL KIMRILETKY
190 200 210 220 230 240
WLEPAGSHGV WGLDDYHFLP FLFGAFQLTT HKHLKPISIH NNELVEMFAH RYLYFGCIAF
250 260 270 280 290 300
INKVKSSASL RWHSPMLDDI SGVKTWSKVA EGMIKMYKAE VLSKLPIMQH FYFSEFLPCP
310 320 330 340 350
DGVSPPRGHI HDGTDKDDEC NFEGHVHTTW GDCCGIKLPS AIAATEMNKK HHKPIPFD
References and
documentation are available.
Number of amino acids: 358
Molecular weight: 41452.45
Theoretical pI: 6.22
Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1880
Hydrogen H 2863
Nitrogen N 489
Oxygen O 535
Sulfur S 18
Formula: C
1880H
2863N
489O
535S
18
Total number of atoms: 5785
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 59735
Abs 0.1% (=1 g/l) 1.441, assuming all pairs of Cys residues form cystines
Ext. coefficient 59360
Abs 0.1% (=1 g/l) 1.432, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.72
This classifies the protein as unstable.
Aliphatic index: 80.34
Grand average of hydropathicity (GRAVY): -0.387