ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLPTFKRYMS SSAHQIPKHF KSLIYSTHEV EDCTKVLSVK NYTPKQDLSQ SIVLKTLAFP 

        70         80         90        100        110        120 
INPSDINQLQ GVYPSRPEKT YDYSTDEPAA IAGNEGVFEV VSLPSGSSKG DLKLGDRVIP 

       130        140        150        160        170        180 
LQANQGTWSN YRVFSSSSDL IKVNDLDLFS AATVSVNGCT GFQLVSDYID WNSNGNEWII 

       190        200        210        220        230        240 
QNAGTSSVSK IVTQVAKAKG IKTLSVIRDR DNFDEVAKVL EDKYGATKVI SESQNNDKTF 

       250        260        270        280        290        300 
AKEVLSKILG ENARVRLALN SVGGKSSASI ARKLENNALM LTYGGMSKQP VTLPTSLHIF 

       310        320        330        340        350        360 
KGLTSKGYWV TEKNKKNPQS KIDTISDFIK MYNYGHIISP RDEIETLTWN TNTTTDEQLL 

       370        380 
ELVKKGITGK GKKKMVVLEW 

References and documentation are available.

Number of amino acids: 380

Molecular weight: 42066.81

Theoretical pI: 9.04

Amino acid composition: 
Ala (A)  19	  5.0%
Arg (R)  10	  2.6%
Asn (N)  24	  6.3%
Asp (D)  21	  5.5%
Cys (C)   2	  0.5%
Gln (Q)  14	  3.7%
Glu (E)  19	  5.0%
Gly (G)  24	  6.3%
His (H)   5	  1.3%
Ile (I)  25	  6.6%
Leu (L)  32	  8.4%
Lys (K)  37	  9.7%
Met (M)   6	  1.6%
Phe (F)  11	  2.9%
Pro (P)  14	  3.7%
Ser (S)  41	 10.8%
Thr (T)  28	  7.4%
Trp (W)   6	  1.6%
Tyr (Y)  13	  3.4%
Val (V)  29	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 47

Atomic composition:

Carbon      C	      1872
Hydrogen    H	      2989
Nitrogen    N	       501
Oxygen      O	       581
Sulfur      S	         8

Formula: C1872H2989N501O581S8
Total number of atoms: 5951

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52495
Abs 0.1% (=1 g/l)   1.248, assuming all pairs of Cys residues form cystines


Ext. coefficient    52370
Abs 0.1% (=1 g/l)   1.245, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.96
This classifies the protein as stable.



Aliphatic index: 85.63

Grand average of hydropathicity (GRAVY): -0.389