ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLQKLSMTAL VGLFSSVVSL VNADCTYSGG NYYCAQTDAI IYSNVGLSAT YQDVTNMDES 

        70         80         90        100        110        120 
SCACTQADFT ASGSLAPFNE ELSVHFRGPI ELLQFGVYYP NGESNALKKR SEKQSIESCK 

       130        140        150        160        170        180 
EGEAVVSRHK HQHKRDVAVE YVQVTSTVYV DSNGQTVTAD STNTVVGPAV PSSYTKDSTV 

       190        200        210        220        230        240 
LSSSAQAVET SESQSSISSS KTTSSAAAAS SSSSSSSNTN GDWSRGSYFV PGSTSNCTFM 

       250        260        270        280        290        300 
NNQGGTAGSG VWSSCFGNSI SFAASDGVSG AASAQALGDV TIKSGNEFMI FSGEECSGNN 

       310        320        330        340        350        360 
GDCGYYREGI PAYHGFGGAD KIFVFEFSMP SDTSGSAYNQ DMPAIWLLNA KIPRTLQYGD 

       370        380        390        400        410        420 
ASCSCWKTGC GEMDLFEILT AGSDKLISHI HDGQDGGTQD YFERPTDGTL KAAVIFNSSD 

       430        440        450 
KTIHIIEVDE SFDATLSDDV VDQWLSKSGS SAALP 

References and documentation are available.

Number of amino acids: 455

Molecular weight: 47994.42

Theoretical pI: 4.49

Amino acid composition: 
Ala (A)  39	  8.6%
Arg (R)   8	  1.8%
Asn (N)  21	  4.6%
Asp (D)  29	  6.4%
Cys (C)  12	  2.6%
Gln (Q)  18	  4.0%
Glu (E)  22	  4.8%
Gly (G)  41	  9.0%
His (H)   8	  1.8%
Ile (I)  19	  4.2%
Leu (L)  23	  5.1%
Lys (K)  17	  3.7%
Met (M)   8	  1.8%
Phe (F)  19	  4.2%
Pro (P)  12	  2.6%
Ser (S)  74	 16.3%
Thr (T)  32	  7.0%
Trp (W)   5	  1.1%
Tyr (Y)  17	  3.7%
Val (V)  31	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      2069
Hydrogen    H	      3170
Nitrogen    N	       556
Oxygen      O	       720
Sulfur      S	        20

Formula: C2069H3170N556O720S20
Total number of atoms: 6535

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53580
Abs 0.1% (=1 g/l)   1.116, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   1.101, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.15
This classifies the protein as unstable.



Aliphatic index: 64.33

Grand average of hydropathicity (GRAVY): -0.253