ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLQLRFMPGW VPRNGFFGLK ETIGTVHKRF YALASEQPSR KTVKPLDSRK TFLIDTYKHL 

        70         80         90        100        110        120 
MENSSMIFFV HYNNLSKTED HHFRFKIKQT GGKLTKVRNN LFEVYLRNSH LPDPCGFVKR 

       130        140        150        160        170        180 
KEQNWKHPLL PLLKGPTATI TYEDTNPQQV AKLLKVLQSA QDKLMVIGAK VENEVLNVEK 

       190        200        210        220        230        240 
INTFKTLPTK PEMQSQLVSV LQMLSGLGLV RTLENSSNAL YLTLKSHNDN QKPKEDVEST 


TDAESKGSK 

References and documentation are available.

Number of amino acids: 249

Molecular weight: 28514.98

Theoretical pI: 9.71

Amino acid composition: 
Ala (A)   8	  3.2%
Arg (R)  10	  4.0%
Asn (N)  16	  6.4%
Asp (D)   9	  3.6%
Cys (C)   1	  0.4%
Gln (Q)  12	  4.8%
Glu (E)  15	  6.0%
Gly (G)  12	  4.8%
His (H)   8	  3.2%
Ile (I)   7	  2.8%
Leu (L)  31	 12.4%
Lys (K)  27	 10.8%
Met (M)   7	  2.8%
Phe (F)  12	  4.8%
Pro (P)  13	  5.2%
Ser (S)  17	  6.8%
Thr (T)  19	  7.6%
Trp (W)   2	  0.8%
Tyr (Y)   6	  2.4%
Val (V)  17	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1280
Hydrogen    H	      2050
Nitrogen    N	       352
Oxygen      O	       368
Sulfur      S	         8

Formula: C1280H2050N352O368S8
Total number of atoms: 4058

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.699, assuming all pairs of Cys residues form cystines


Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.699, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.21
This classifies the protein as stable.



Aliphatic index: 82.53

Grand average of hydropathicity (GRAVY): -0.544