ProtParam
User-provided sequence:
10 20 30 40 50 60
MLQQGSSSRR SLHGNDFHTL TSPSRRDSLS IPRAVDARSA STIDLFYIPD ATVSRRHSTL
70 80 90 100 110 120
VANRSDNNGN GAPMRQYNKP NFASSSTSSL PSTRNRPSRY DNMNMNMNMN MNMNMNMNMN
130 140 150 160 170 180
MNNHTTSDHN AHPQYRCRPN PSRRHSLMTI PEKYSGSRYS LRSSPPTYSN PRVRKELTPF
190 200 210 220 230 240
QLQRKQMKSA FQFPNGENFT PRNQIARLPP SSTFPDSPSS SSLPLTQTGG PSSADNDSIA
250 260 270 280 290 300
TGTNNRSPQQ TKAADANQKS ESESPKAIRS NSKKISRFFR KIWSSKSSNS ADSVEENSKT
310 320 330 340 350 360
KQKRKNPERV VPEPITSLDQ PVEIIKQSFS TVNNHETAVP SIKDSGIVQE LTALGDNNRI
370 380 390 400 410 420
PVLPPPRSPN RPTLSDKRTT KLYYCSQDSS NEDIAPEEKS TVFLKRLQDE WSTVYLNKLP
430 440 450 460 470 480
LTASVPSSLS TTTDAANSSF INSSISSPAP SSSSSSSLVS RGPMQSISSS PTPAPSSGSS
490 500 510 520 530 540
KSKNAVKSLR FADEIYVNDT WSAADYCRCD NTFLNNFFKG KSQDITNPST FVGNNLSSTK
550 560 570
NISNIEIKME VNEFKRKEMR VHQDSAKYTH YYL
References and
documentation are available.
Number of amino acids: 573
Molecular weight: 63711.60
Theoretical pI: 9.86
Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 73
Atomic composition:
Carbon C 2721
Hydrogen H 4335
Nitrogen N 825
Oxygen O 902
Sulfur S 21
Formula: C
2721H
4335N
825O
902S
21
Total number of atoms: 8804
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39100
Abs 0.1% (=1 g/l) 0.614, assuming all pairs of Cys residues form cystines
Ext. coefficient 38850
Abs 0.1% (=1 g/l) 0.610, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 66.37
This classifies the protein as unstable.
Aliphatic index: 53.30
Grand average of hydropathicity (GRAVY): -0.911