ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLQRRFISSS GIKRLLHRES NKVMHTVFFK VRYYSTELIK KKHKEDIEDW VKAQLKDSST 

        70         80         90        100        110        120 
ISGVYESRNK LDWMDSITKS PSSLDILKNQ YNIVKDKDFG ILWKQKFESA DPDILMTIIS 

       130        140        150        160        170        180 
LSTNQKVLFS IQQLLILINS LHFLKRDYDI GQIYTTYEQF TPLLASHTDK GTYGQFIEIM 

       190        200        210        220        230        240 
LVVQHNLHHF DVCETLFAEY IKYCKVKPQM ISLGLNSFIR SNNTQLAVEF YTQAITNPDT 

       250        260        270        280        290        300 
FPITEKQLFE FLRCMERYLD MSSMKHIFYL WLKVKCGDEQ SSSTNLPSFK TLAIIHRMLL 

       310        320        330        340        350        360 
RFSNTDELND FLTNPVVLST GYTSSVQFEL IEFCHSLYCI KGDRTKSIDD SILMERVDKF 

       370        380        390        400        410        420 
ITRLNNNIST RKELYMSVVQ AYVSTNNFEN LKVILEKIQR DNDISIDGSF HLCISRYFVN 

       430        440        450        460        470        480 
TNQFEGLFKY YRSVVKTTDG KTRLRPAFIQ QLWSCAVNVY PMLAKEITND LLVTLKRSQY 

       490        500        510        520        530        540 
SKCLTWVYTF LQENAHIHTR KINGGEDSSL SGFNAVDFER FEEFKKKVSH NDVYGAELVI 

       550        560        570        580        590        600 
SNSLKEGIAP QFSFLYSVLA LCLRNSLTGL ARVVDVILRT RFRYIPLKVD ILWLKWEIIS 

       610        620        630        640        650        660 
NYRSFEKLSA EHLKELEFKL KEFERVHQKE LSVQNYLQLT QICFHTRDFK YACYLISQAR 

       670        680        690        700        710        720 
KNLDTSNNKQ WMMYYMTSLK LASRMHESER FSRILKDWNC NHRASLITPG CIRQIKGFMK 

       730        740        750        760        770        780 
YFEKRPAYIS TAASIDNKEI KDRIDELVLR YVDYKYQGLE NMRKLTLFLK EWFDEEISLL 

       790        800 
KLEQNERKMK LFEENKKEEE 

References and documentation are available.

Number of amino acids: 800

Molecular weight: 94523.07

Theoretical pI: 8.94

Amino acid composition: 
Ala (A)  25	  3.1%
Arg (R)  43	  5.4%
Asn (N)  43	  5.4%
Asp (D)  41	  5.1%
Cys (C)  14	  1.8%
Gln (Q)  34	  4.2%
Glu (E)  55	  6.9%
Gly (G)  22	  2.8%
His (H)  20	  2.5%
Ile (I)  60	  7.5%
Leu (L)  90	 11.2%
Lys (K)  69	  8.6%
Met (M)  20	  2.5%
Phe (F)  48	  6.0%
Pro (P)  14	  1.8%
Ser (S)  68	  8.5%
Thr (T)  45	  5.6%
Trp (W)  11	  1.4%
Tyr (Y)  37	  4.6%
Val (V)  41	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 112

Atomic composition:

Carbon      C	      4279
Hydrogen    H	      6694
Nitrogen    N	      1126
Oxygen      O	      1220
Sulfur      S	        34

Formula: C4279H6694N1126O1220S34
Total number of atoms: 13353

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   116505
Abs 0.1% (=1 g/l)   1.233, assuming all pairs of Cys residues form cystines


Ext. coefficient   115630
Abs 0.1% (=1 g/l)   1.223, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.89
This classifies the protein as stable.



Aliphatic index: 91.11

Grand average of hydropathicity (GRAVY): -0.338