ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRAASNGLR NTVAARSVSL SAANHSDAKR SDIAQYKVVD HAYDAVVVGA GGAGLRAAMG
70 80 90 100 110 120
LAEGGLKTAV ITKLFPTRSH TVAAQGGINA ALGNMNPDNW RWHFYDTVKG SDWLGDQDAI
130 140 150 160 170 180
HYMTREAERA VIELENYGMP FSRTTDGKIY QRAFGGQSND FGRGGQAHRT CCVADRTGHS
190 200 210 220 230 240
LLHTLYGASL QYNCNYFVEY FALDLIMENG VCVGVIAMDL EDGTIHRFRS KNTVLATGGY
250 260 270 280 290 300
GRAFFSCTSA HTCTGDGTAL TARAGINNSD MEFVQFHPTG IYGAGCLITE GSRGEGGYLV
310 320 330 340 350 360
NSAGERFMER YAPNAKDLAS RDVVSRSMTV EIMEGRGVGP DKDHIYLQLH HLPAEQLQQR
370 380 390 400 410 420
LPGISETAMI FAGVDVTKEP IPVIPTVHYN MGGVPTNYKG QVLNYTPKKG DEVVPGLYAA
430 440 450 460 470 480
GECGAHSVHG ANRLGANSLL DLVIFGRACA IDILKNTSAG VGVPELPKNA GEASVANIDK
490 500 510 520 530 540
LRHNKGDIST AELRLTMQKS MQNHAAVFRR GDILKEGVKV LSKLYKDQAH LNVADKGLVW
550 560 570 580 590 600
NSDLIETLEL QNLLINATQT IVAAENREES RGAHARDDFP DRLDELDYSK PLEGQTKKEL
610 620 630 640
KDHWRKHSII RSNIETGEVS LDYRPVIDTT LDKSETDWVP PKVRSY
References and
documentation are available.
Number of amino acids: 646
Molecular weight: 70398.30
Theoretical pI: 6.37
Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 3078
Hydrogen H 4857
Nitrogen N 897
Oxygen O 950
Sulfur S 24
Formula: C
3078H
4857N
897O
950S
24
Total number of atoms: 9806
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67770
Abs 0.1% (=1 g/l) 0.963, assuming all pairs of Cys residues form cystines
Ext. coefficient 67270
Abs 0.1% (=1 g/l) 0.956, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.60
This classifies the protein as stable.
Aliphatic index: 81.87
Grand average of hydropathicity (GRAVY): -0.331