ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLREMLGCCK VYISEARNKT ALEAIERALK PFPPAAIVNK FEDAAYGRVG YTVVSSLANG 

        70         80         90        100        110        120 
SSSSLKNAVF AMVKTALDTI NLELHCGSHP RLGVVDHICF HPLSQTSIEQ VSSVANSLAM 

       130        140        150        160        170        180 
DIGSILRVPT YLYGAAEKEQ CTLDSIRRKL GYFKANREGH EWAGGFDLEM VPLKPDAGPQ 

       190        200        210        220        230        240 
EVSKAKGVVA VGACGWVSNY NVPVMSNDLK AVRRIARKTS ERGGGLASVQ TMALVHGEGV 

       250        260        270        280        290 
IEVACNLLNP SQVGGDEVQG LIERLGREEG LLVGKGYYTD YTPDQIVERY MDLLNNS 

References and documentation are available.

Number of amino acids: 297

Molecular weight: 32136.79

Theoretical pI: 6.33

Amino acid composition: 
Ala (A)  27	  9.1%
Arg (R)  16	  5.4%
Asn (N)  14	  4.7%
Asp (D)  12	  4.0%
Cys (C)   7	  2.4%
Gln (Q)   8	  2.7%
Glu (E)  21	  7.1%
Gly (G)  28	  9.4%
His (H)   6	  2.0%
Ile (I)  13	  4.4%
Leu (L)  29	  9.8%
Lys (K)  15	  5.1%
Met (M)   8	  2.7%
Phe (F)   6	  2.0%
Pro (P)  12	  4.0%
Ser (S)  22	  7.4%
Thr (T)  11	  3.7%
Trp (W)   2	  0.7%
Tyr (Y)  11	  3.7%
Val (V)  29	  9.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1411
Hydrogen    H	      2264
Nitrogen    N	       396
Oxygen      O	       430
Sulfur      S	        15

Formula: C1411H2264N396O430S15
Total number of atoms: 4516

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27765
Abs 0.1% (=1 g/l)   0.864, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.852, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.89
This classifies the protein as stable.



Aliphatic index: 92.56

Grand average of hydropathicity (GRAVY): -0.086