ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRFTGARAI RKYSTRYALE HLKEGAPLKG LFSIEGLQKA WFDRVKYLDA KLNDCTNEAQ 

        70         80         90        100        110        120 
QKPLETLIHE NSKSASKKHI VNYASSLYNL KFSMSSLQGC IRTPPEECPR LGPEALLQTP 

       130        140        150        160        170        180 
DFNRTISNEP LTTGNERLQA ALISSFGSLM EFRTLLINSN LAISGDGFTW LVARRQLDKR 

       190        200        210        220        230        240 
AMRNDMPNRD IEYDKLFILN TYNAGTPFNF STSGVMNELN NQYTNMEKQR AKEAGNLEDS 

       250        260        270        280        290        300 
EMTAKQAKTK FIYETQQKGF SGKEVSYIPL LAIDASPKTW LTDYGVFGKR EYLERVWDSI 

       310        320 
EWKIVESRLP QRTKIQAFNT L 

References and documentation are available.

Number of amino acids: 321

Molecular weight: 36728.83

Theoretical pI: 9.13

Amino acid composition: 
Ala (A)  23	  7.2%
Arg (R)  20	  6.2%
Asn (N)  21	  6.5%
Asp (D)  13	  4.0%
Cys (C)   3	  0.9%
Gln (Q)  14	  4.4%
Glu (E)  24	  7.5%
Gly (G)  17	  5.3%
His (H)   3	  0.9%
Ile (I)  17	  5.3%
Leu (L)  35	 10.9%
Lys (K)  24	  7.5%
Met (M)   8	  2.5%
Phe (F)  15	  4.7%
Pro (P)  13	  4.0%
Ser (S)  24	  7.5%
Thr (T)  22	  6.9%
Trp (W)   5	  1.6%
Tyr (Y)  12	  3.7%
Val (V)   8	  2.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1635
Hydrogen    H	      2577
Nitrogen    N	       451
Oxygen      O	       489
Sulfur      S	        11

Formula: C1635H2577N451O489S11
Total number of atoms: 5163

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45505
Abs 0.1% (=1 g/l)   1.239, assuming all pairs of Cys residues form cystines


Ext. coefficient    45380
Abs 0.1% (=1 g/l)   1.236, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.35
This classifies the protein as stable.



Aliphatic index: 77.57

Grand average of hydropathicity (GRAVY): -0.555