ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRFTNCSCK TFVKSSYKLN IRRMNSSFRT ETDAFGEIHV PADKYWGAQT QRSFQNFKIG 

        70         80         90        100        110        120 
GARERMPLPL VHAFGVLKKS AAIVNESLGG LDPKISKAIQ QAADEVASGK LDDHFPLVVF 

       130        140        150        160        170        180 
QTGSGTQSNM NANEVISNRA IEILGGKIGS KQVHPNNHCN QSQSSNDTFP TVMHIAASLQ 

       190        200        210        220        230        240 
IQNELIPELT NLKNALEAKS KEFDHIVKIG RTHLQDATPL TLGQEFSGYV QQVENGIQRV 

       250        260        270        280        290        300 
AHSLKTLSFL AQGGTAVGTG LNTKPGFDVK IAEQISKETG LKFQTAPNKF EALAAHDAIV 

       310        320        330        340        350        360 
ECSGALNTLA CSLFKIAQDI RYLGSGPRCG YHELMLPENE PGSSIMPGKV NPTQNEALTQ 

       370        380        390        400        410        420 
VCVQVMGNNA AITFAGSQGQ FELNVFKPVM IANLLNSIRL ITDAAYSFRV HCVEGIKANE 

       430        440        450        460        470        480 
PRIHELLTKS LMLVTALNPK IGYDAASKVA KNAHKKGITL KESALELGVL TEKEFDEWVV 


PEHMLGPK 

References and documentation are available.

Number of amino acids: 488

Molecular weight: 53151.84

Theoretical pI: 8.48

Amino acid composition: 
Ala (A)  44	  9.0%
Arg (R)  15	  3.1%
Asn (N)  31	  6.4%
Asp (D)  15	  3.1%
Cys (C)   8	  1.6%
Gln (Q)  26	  5.3%
Glu (E)  31	  6.4%
Gly (G)  37	  7.6%
His (H)  15	  3.1%
Ile (I)  30	  6.1%
Leu (L)  45	  9.2%
Lys (K)  35	  7.2%
Met (M)  11	  2.3%
Phe (F)  22	  4.5%
Pro (P)  21	  4.3%
Ser (S)  34	  7.0%
Thr (T)  28	  5.7%
Trp (W)   2	  0.4%
Tyr (Y)   7	  1.4%
Val (V)  31	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2351
Hydrogen    H	      3761
Nitrogen    N	       657
Oxygen      O	       707
Sulfur      S	        19

Formula: C2351H3761N657O707S19
Total number of atoms: 7495

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21930
Abs 0.1% (=1 g/l)   0.413, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.403, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.98
This classifies the protein as stable.



Aliphatic index: 87.38

Grand average of hydropathicity (GRAVY): -0.206