ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRFVSSQTC RYSSRGLLKT SLLKNASTVK IVGRGLATTG TDNFLSTSNA TYIDEMYQAW 

        70         80         90        100        110        120 
QKDPSSVHVS WDAYFKNMSN PKIPATKAFQ APPSISNFPQ GTEAAPLGTA MTGSVDENVS 

       130        140        150        160        170        180 
IHLKVQLLCR AYQVRGHLKA HIDPLGISFG SNKNNPVPPE LTLDYYGFSK HDLDKEINLG 

       190        200        210        220        230        240 
PGILPRFARD GKSKMSLKEI VDHLEKLYCS SYGVQYTHIP SKQKCDWLRE RIEIPEPYQY 

       250        260        270        280        290        300 
TVDQKRQILD RLTWATSFES FLSTKFPNDK RFGLEGLESV VPGIKTLVDR SVELGVEDIV 

       310        320        330        340        350        360 
LGMAHRGRLN VLSNVVRKPN ESIFSEFKGS SARDDIEGSG DVKYHLGMNY QRPTTSGKYV 

       370        380        390        400        410        420 
NLSLVANPSH LESQDPVVLG RTRALLHAKN DLKEKTKALG VLLHGDAAFA GQGVVYETMG 

       430        440        450        460        470        480 
FLTLPEYSTG GTIHVITNNQ IGFTTDPRFA RSTPYPSDLA KAIDAPIFHV NANDVEAVTF 

       490        500        510        520        530        540 
IFNLAAEWRH KFHTDAIIDV VGWRKHGHNE TDQPSFTQPL MYKKIAKQKS VIDVYTEKLI 

       550        560        570        580        590        600 
SEGTFSKKDI DEHKKWVWNL FEDAFEKAKD YVPSQREWLT AAWEGFKSPK ELATEILPHE 

       610        620        630        640        650        660 
PTNVPESTLK ELGKVLSSWP EGFEVHKNLK RILKNRGKSI ETGEGIDWAT GEALAFGTLV 

       670        680        690        700        710        720 
LDGQNVRVSG EDVERGTFSQ RHAVLHDQQS EAIYTPLSTL NNEKADFTIA NSSLSEYGVM 

       730        740        750        760        770        780 
GFEYGYSLTS PDYLVMWEAQ FGDFANTAQV IIDQFIAGGE QKWKQRSGLV LSLPHGYDGQ 

       790        800        810        820        830        840 
GPEHSSGRLE RFLQLANEDP RYFPSEEKLQ RQHQDCNFQV VYPTTPANLF HILRRQQHRQ 

       850        860        870        880        890        900 
FRKPLALFFS KQLLRHPLAR SSLSEFTEGG FQWIIEDIEH GKSIGTKEET KRLVLLSGQV 

       910        920        930        940        950        960 
YTALHKRRES LGDKTTAFLK IEQLHPFPFA QLRDSLNSYP NLEEIVWCQE EPLNMGSWAY 

       970        980        990       1000       1010 
TEPRLHTTLK ETDKYKDFKV RYCGRNPSGA VAAGSKSLHL AEEDAFLKDV FQQS 

References and documentation are available.

Number of amino acids: 1014

Molecular weight: 114416.30

Theoretical pI: 6.74

Amino acid composition: 
Ala (A)  63	  6.2%
Arg (R)  49	  4.8%
Asn (N)  41	  4.0%
Asp (D)  53	  5.2%
Cys (C)   7	  0.7%
Gln (Q)  46	  4.5%
Glu (E)  71	  7.0%
Gly (G)  70	  6.9%
His (H)  34	  3.4%
Ile (I)  46	  4.5%
Leu (L)  98	  9.7%
Lys (K)  70	  6.9%
Met (M)  12	  1.2%
Phe (F)  50	  4.9%
Pro (P)  51	  5.0%
Ser (S)  80	  7.9%
Thr (T)  62	  6.1%
Trp (W)  17	  1.7%
Tyr (Y)  34	  3.4%
Val (V)  60	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 124
Total number of positively charged residues (Arg + Lys): 119

Atomic composition:

Carbon      C	      5139
Hydrogen    H	      7953
Nitrogen    N	      1403
Oxygen      O	      1526
Sulfur      S	        19

Formula: C5139H7953N1403O1526S19
Total number of atoms: 16040

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   144535
Abs 0.1% (=1 g/l)   1.263, assuming all pairs of Cys residues form cystines


Ext. coefficient   144160
Abs 0.1% (=1 g/l)   1.260, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.22
This classifies the protein as stable.



Aliphatic index: 78.76

Grand average of hydropathicity (GRAVY): -0.485