ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRGRSLSVT SLGGLPAWEA ERLPVEDLLL FEVSWEVTNK VGGICTVIQS KAKTTANEWG 

        70         80         90        100        110        120 
ENYFLIGPYF EHNVKTQVEP CEPANDAVRK AVDAMNKHGC QVHFGRWLIE GSPYVVLFDI 

       130        140        150        160        170        180 
SSSVWNLDRW KGDFWEACGV GIPHDDREAN DMLIFGSLTA WFLKEVTDHA DGKHVIAQFH 

       190        200        210        220        230        240 
EWQAGTGLIL SRARKLPIAT IFTTHATLLG RYLCAANIDF YNQLDKFNID KEAGERQIYH 

       250        260        270        280        290        300 
RYCMERASVH CAHVFTTVSE ITAIEADHML KRKPDVVTPN GLNVKKFSAV HEFQNLHATY 

       310        320        330        340        350        360 
KARIQDFVRG HFYGHLDFDL EKTLFLFIAG RYEFSNKGAD IFLESLSRLN FLLRMHKSNV 

       370        380        390        400        410        420 
TVVVFFIMPA KTNNFNVETL KGQAVRKQLW DTVHCMKEKF GKKLYDGLLR GEIPDMNSIL 

       430        440        450        460        470        480 
DRDDLTIMKR AIFSTQRHSL PPVTTHNMID DSTDPILSTI RRIGLFNNRT DRVKVILHPE 

       490        500        510        520        530        540 
FLSSTSPLLP MDYEEFVRGC HLGVFPSYYE PWGYTPAECT VMGIPSVTTN LSGFGCFMQE 

       550        560        570        580        590        600 
HVADPTAYGI YIVDRRFRSP DDSCNQLTQF LYGFCKQSRR QRIIQRNRTE RLSDLLDWRY 

       610        620        630        640        650        660 
LGRYYQHARH LTLSRAFPDK FHLEPTSPPT TDGFKYPRPS SVPPSPSGSQ TSSPQSSDVE 

       670        680        690        700 
NEGDEDERYD EEEEAERDRL NIKSPFSLNH IPKGKKKLHG EYKN 

References and documentation are available.

Number of amino acids: 704

Molecular weight: 80733.66

Theoretical pI: 6.64

Amino acid composition: 
Ala (A)  38	  5.4%
Arg (R)  46	  6.5%
Asn (N)  31	  4.4%
Asp (D)  44	  6.2%
Cys (C)  13	  1.8%
Gln (Q)  21	  3.0%
Glu (E)  45	  6.4%
Gly (G)  43	  6.1%
His (H)  28	  4.0%
Ile (I)  36	  5.1%
Leu (L)  62	  8.8%
Lys (K)  38	  5.4%
Met (M)  14	  2.0%
Phe (F)  41	  5.8%
Pro (P)  36	  5.1%
Ser (S)  46	  6.5%
Thr (T)  43	  6.1%
Trp (W)  12	  1.7%
Tyr (Y)  24	  3.4%
Val (V)  43	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 89
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      3621
Hydrogen    H	      5558
Nitrogen    N	      1000
Oxygen      O	      1048
Sulfur      S	        27

Formula: C3621H5558N1000O1048S27
Total number of atoms: 11254

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   102510
Abs 0.1% (=1 g/l)   1.270, assuming all pairs of Cys residues form cystines


Ext. coefficient   101760
Abs 0.1% (=1 g/l)   1.260, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.74
This classifies the protein as unstable.



Aliphatic index: 77.40

Grand average of hydropathicity (GRAVY): -0.428