ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRISIDSIK QFGSFVTGYN NTSYHAAGRA IRTSSLYSTM ISANPRRCLH SSKLLNKEGQ 

        70         80         90        100        110        120 
EEGYNEQLIS KMSSQNGSNS RQNESEGKKE GKASSVKSLL QHTHSHSHTH MHDNPLLSLN 

       130        140        150        160        170        180 
VQQIKKNPGV RITWIGLASN VGMAVGKFVG GITFHSQALL ADSVHALSDL VSDFLTLFSV 

       190        200        210        220        230        240 
QYASRKPTSE YPYGYGKVET VGSLAVSTIL AMAGISIGWS SLCAIVGPVI PHAILESMAG 

       250        260        270        280        290        300 
LIGETHSHSQ SLTQQATNVN AVWIAAGSIL VKEWVFQATK KVAIQTNSNV LMANAWHHRV 

       310        320        330        340        350        360 
DSLTSLVALV AITSSYFFNI QSLDNLGGLV VSGLIIKTGG QGILSSLKEL VDQSIPPTDP 

       370        380        390        400        410        420 
RYLEIESVIK DSIGSLKTDL DLKQSLHVRD LTILASGPNL RATTTLEVPV LHSGQEVGIR 

       430        440 
FLENAISTIR EDLRMKVPNV GRWTSSLLM 

References and documentation are available.

Number of amino acids: 449

Molecular weight: 48610.53

Theoretical pI: 9.01

Amino acid composition: 
Ala (A)  30	  6.7%
Arg (R)  16	  3.6%
Asn (N)  22	  4.9%
Asp (D)  14	  3.1%
Cys (C)   2	  0.4%
Gln (Q)  20	  4.5%
Glu (E)  19	  4.2%
Gly (G)  34	  7.6%
His (H)  17	  3.8%
Ile (I)  33	  7.3%
Leu (L)  50	 11.1%
Lys (K)  22	  4.9%
Met (M)  10	  2.2%
Phe (F)  10	  2.2%
Pro (P)  13	  2.9%
Ser (S)  59	 13.1%
Thr (T)  28	  6.2%
Trp (W)   6	  1.3%
Tyr (Y)  10	  2.2%
Val (V)  34	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      2151
Hydrogen    H	      3465
Nitrogen    N	       601
Oxygen      O	       655
Sulfur      S	        12

Formula: C2151H3465N601O655S12
Total number of atoms: 6884

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48025
Abs 0.1% (=1 g/l)   0.988, assuming all pairs of Cys residues form cystines


Ext. coefficient    47900
Abs 0.1% (=1 g/l)   0.985, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.21
This classifies the protein as stable.



Aliphatic index: 100.73

Grand average of hydropathicity (GRAVY): -0.016