ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRNCLRKLG NHQTKCSVKT LHTPIYRTKN LQVLRDTLSG IKLLEKIITS SSYNKTLIYE
70 80 90 100 110 120
PKYKSKPQVV SSHDTMRLHN VMRELLDSLQ VDEATNTRLQ SNRPRKLGRV GLQLFMDCIQ
130 140 150 160 170 180
DNLTATSTSL TCSLLEHYFK YPEKEVTNGI KAGLRYIRDF LAKNKIIVKS QNDVDALVEQ
190 200 210 220 230 240
LTMSSSDSQS IKRVLKAINY ELFSDDIVRV INGNKTYDEV DVSKGWKYPA GILDSNEAYL
250 260 270 280 290 300
RSLELPTKKL VSIDKDMLVL MYDGTLRDAN KILPTITYAR KLRKSVLLIV NGDCTGDALT
310 320 330 340 350 360
SVTINNNRNK RENNESRIVV LKYSKKANND LAPQENLDFI KFLRLPCGYD SIYSPEYSPL
370 380 390 400 410 420
VPSKMCADKY YGSIESIKAT TGEAFLYNSI DAEAIPNKVP KSFLQNTVTL SIGGHNEIEI
430 440 450 460 470 480
DRRRNAIDNC LNNVLCHGLA KGFIPGYGIS LLKAIPGLNE LKANEPNFMT KVGINAVLSA
490 500 510 520 530 540
VILPSEVAFK NAYGYNYYEI NSLIAGAINE KSFPMAKFSP NSEPVNTVKD GNLEPWSKMD
550 560 570
SCLAGVETFI ELLTSCNTII TCVYKSLNGT KPR
References and
documentation are available.
Number of amino acids: 573
Molecular weight: 64258.98
Theoretical pI: 9.05
Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 2850
Hydrogen H 4607
Nitrogen N 775
Oxygen O 862
Sulfur S 23
Formula: C
2850H
4607N
775O
862S
23
Total number of atoms: 9117
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 50490
Abs 0.1% (=1 g/l) 0.786, assuming all pairs of Cys residues form cystines
Ext. coefficient 49740
Abs 0.1% (=1 g/l) 0.774, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.01
This classifies the protein as stable.
Aliphatic index: 95.78
Grand average of hydropathicity (GRAVY): -0.293