ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRNLVVRNA CRNRPSIQVA RGLCRHQTRR LMASSPQFGR NSNQEKTAGF IMGILSMAGA
70 80 90 100 110 120
LYFIAPNRKP LFASRKVESD KTAEEELSSG GEQSPENEDD NNSKSDENGD DNDSKNDETE
130 140 150 160 170 180
AGPQLGGDKI GASKVAEDGE LVVLAEEDNK SSEDKDTDES KVSTKDDEQS NEDNATANNQ
190 200 210 220 230 240
KDENISSENS EENTSDKTLD NNAGSSEKKD PEHSDDEKSQ QGQSDDKTTT EDNNGEEESS
250 260 270 280 290 300
KKTVSDSENS AKQSESSDEE KEELRKQEEK QMGPTEEEVQ HEGAYNPDTG EINWDCPCLG
310 320 330 340 350 360
GMAHGPCGEE FKSAFSCFVY SEAEPKGIDC VEKFQHMQDC FRKYPEHYAE QLKETSDDEE
370 380 390 400
PQDKVKVNTI ESAPNVSSAK ENAAKKAEQS DVKKEPLNEE SKP
References and
documentation are available.
Number of amino acids: 403
Molecular weight: 44536.49
Theoretical pI: 4.46
Total number of negatively charged residues (Asp + Glu): 95
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 1842
Hydrogen H 2911
Nitrogen N 543
Oxygen O 712
Sulfur S 15
Formula: C
1842H
2911N
543O
712S
15
Total number of atoms: 6023
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13450
Abs 0.1% (=1 g/l) 0.302, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.291, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 60.59
This classifies the protein as unstable.
Aliphatic index: 42.41
Grand average of hydropathicity (GRAVY): -1.364