ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRNTIALRS FIRTQSTRPY PVNVEAVYYA PLKLPIKYGD LVADIQLRSY DNENLDFYSD
70 80 90 100 110 120
FILRTGYYLG IPLTGPKPLP TRRERWTVIK SPFVHAKSKE NFERHTHKRL IRAWDTNPEV
130 140 150 160 170 180
LQMLIAYITK HSMAGVGMKC NFFQRSEISL DLGSDANGLE KSLSNIDELY SLRNDDKAQT
190 200
SAVGQKVLEL LDSPDFKKHL EKK
References and
documentation are available.
Number of amino acids: 203
Molecular weight: 23423.90
Theoretical pI: 9.30
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1055
Hydrogen H 1667
Nitrogen N 289
Oxygen O 304
Sulfur S 5
Formula: C
1055H
1667N
289O
304S
5
Total number of atoms: 3320
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 1.106, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 1.106, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.04
This classifies the protein as unstable.
Aliphatic index: 88.87
Grand average of hydropathicity (GRAVY): -0.481