ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRQGAGTLQ LVSQGFANAE QARGFATLKD ISIRLKSVKN IQKITKSMKM VAAAKYAKAE 

        70         80         90        100        110        120 
RELKGARAYG VGAKTFFDNI DPVVEGVEKQ ESKKQVLVLI TSDRGLCGGV HSSIVKEAKN 

       130        140        150        160        170        180 
ILNNAGDKEI RVVAIGDKSR AGLQRLYANS ILLSGNEIGR APPSFADASI AAKAILDSGY 

       190        200        210        220        230        240 
DFETGTILFN RFKTVVSYET SKLQILPLEA IKAKEALSTY DSVDDDVLQS YSEYSLAQLI 

       250        260        270        280        290        300 
YYGMKESATS ELPASKYLSL SVPFLHPLLF KLSLFRRPNF PHFKSFLSPP KIRCFDLFFI 

       310 
FLCVTRCYSD TFE 

References and documentation are available.

Number of amino acids: 313

Molecular weight: 34601.99

Theoretical pI: 9.29

Amino acid composition: 
Ala (A)  30	  9.6%
Arg (R)  15	  4.8%
Asn (N)  10	  3.2%
Asp (D)  15	  4.8%
Cys (C)   4	  1.3%
Gln (Q)  11	  3.5%
Glu (E)  17	  5.4%
Gly (G)  19	  6.1%
His (H)   3	  1.0%
Ile (I)  20	  6.4%
Leu (L)  34	 10.9%
Lys (K)  27	  8.6%
Met (M)   4	  1.3%
Phe (F)  19	  6.1%
Pro (P)  11	  3.5%
Ser (S)  30	  9.6%
Thr (T)  13	  4.2%
Trp (W)   0	  0.0%
Tyr (Y)  12	  3.8%
Val (V)  19	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1565
Hydrogen    H	      2494
Nitrogen    N	       412
Oxygen      O	       454
Sulfur      S	         8

Formula: C1565H2494N412O454S8
Total number of atoms: 4933

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18130
Abs 0.1% (=1 g/l)   0.524, assuming all pairs of Cys residues form cystines


Ext. coefficient    17880
Abs 0.1% (=1 g/l)   0.517, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.60
This classifies the protein as stable.



Aliphatic index: 94.47

Grand average of hydropathicity (GRAVY): -0.058