ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRQVKPKNA RTKRALEKRE PKLVEGAKTA IFLRGNATSQ ISLDVLGDIH ALKKPFSVNF
70 80 90 100 110 120
QKKNNILPFE DASSLEFFSE KNDAALAVMA THNKKRPHNL TWVRFFNYRV LDMIELGIVN
130 140 150 160 170 180
YKSIQSFSAT PIVPGTKPMI LFQGPVFDAH PTYRHIKSLF LDFFRGEPIQ KLDSAGLSYV
190 200 210 220 230 240
IVVSAAEAQE DETKPLPLVH FRVYGTKLLK TKTNLPRVEL EEMGPRIDFN IRRVQPAESD
250 260 270 280 290 300
VLEEALKKPK TQEPKPKKNV DVDIIGNKVG RIHVDQQDLG NLQTRKMKGL KRSVEEREDS
310
ENEEVEIEED VISDASE
References and
documentation are available.
Number of amino acids: 317
Molecular weight: 36071.46
Theoretical pI: 8.96
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 1614
Hydrogen H 2598
Nitrogen N 448
Oxygen O 475
Sulfur S 6
Formula: C
1614H
2598N
448O
475S
6
Total number of atoms: 5141
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.359
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.26
This classifies the protein as unstable.
Aliphatic index: 88.23
Grand average of hydropathicity (GRAVY): -0.513