ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRQVLRRSL SDSRRPLRSV GSISCSARAS GIGMRAYSSQ IFKEGMQLET HTDIQSAARE 

        70         80         90        100        110        120 
QAQERMGSEA LASNNSVLKH AYQESVDHGT RPIYLDMQAT TPTDPRVVDT MLKFYTGLYG 

       130        140        150        160        170        180 
NPHSNTHSYG WETSQEVEKA RKNVADVIKA DPKEIIFTSG ATESNNMALK GVARFYKKRK 

       190        200        210        220        230        240 
NHIITTRTEH KCVLEAARSM KDEGFDVTFL NVNEDGLVSL EELEQAIRPE TSLVSVMSVN 

       250        260        270        280        290        300 
NEIGVVQPIK EIGAICRRHK VFFHSDAAQA YGKIPIDVDE MNIDLLSISS HKIYGPKGIG 

       310        320        330        340        350        360 
ALYVRRRPRV RMEPLLSGGG QERGFRSGTL PPPLVVGLGH AAKLMVEEYE YDSAHVRRLS 

       370        380        390        400        410        420 
DRLLKGLLSI EQTTLNGSAD HRYPGCVNVS FAFVEGESLL MALRDIALSS GSACTSASLE 

       430        440        450        460        470        480 
PSYVLHAIGR DDALAHSSIR FGIGRFTTEA EVDYVIKAIT ERVEFLRELS PLWEMYKDGI 

       490 
DLNTIEWSGH 

References and documentation are available.

Number of amino acids: 490

Molecular weight: 54435.78

Theoretical pI: 6.62

Amino acid composition: 
Ala (A)  38	  7.8%
Arg (R)  37	  7.6%
Asn (N)  16	  3.3%
Asp (D)  24	  4.9%
Cys (C)   5	  1.0%
Gln (Q)  14	  2.9%
Glu (E)  38	  7.8%
Gly (G)  36	  7.3%
His (H)  16	  3.3%
Ile (I)  30	  6.1%
Leu (L)  43	  8.8%
Lys (K)  22	  4.5%
Met (M)  14	  2.9%
Phe (F)  14	  2.9%
Pro (P)  18	  3.7%
Ser (S)  47	  9.6%
Thr (T)  25	  5.1%
Trp (W)   3	  0.6%
Tyr (Y)  16	  3.3%
Val (V)  34	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2383
Hydrogen    H	      3810
Nitrogen    N	       688
Oxygen      O	       733
Sulfur      S	        19

Formula: C2383H3810N688O733S19
Total number of atoms: 7633

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40590
Abs 0.1% (=1 g/l)   0.746, assuming all pairs of Cys residues form cystines


Ext. coefficient    40340
Abs 0.1% (=1 g/l)   0.741, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.55
This classifies the protein as unstable.



Aliphatic index: 85.98

Grand average of hydropathicity (GRAVY): -0.325