ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRSSVVRSR ATLRPLLRRA YSSHKELKFG VEGRASLLKG VETLAEAVAA TLGPKGRNVL
70 80 90 100 110 120
IEQPFGPPKI TKDGVTVAKS IVLKDKFENM GAKLLQEVAS KTNEAAGDGT TSATVLGRAI
130 140 150 160 170 180
FTESVKNVAA GCNPMDLRRG SQVAVEKVIE FLSANKKEIT TSEEIAQVAT ISANGDSHVG
190 200 210 220 230 240
KLLASAMEKV GKEGVITIRE GRTLEDELEV TEGMRFDRGF ISPYFITDPK SSKVEFEKPL
250 260 270 280 290 300
LLLSEKKISS IQDILPALEI SNQSRRPLLI IAEDVDGEAL AACILNKLRG QVKVCAVKAP
310 320 330 340 350 360
GFGDNRKNTI GDIAVLTGGT VFTEELDLKP EQCTIENLGS CDSITVTKED TVILNGSGPK
370 380 390 400 410 420
EAIQERIEQI KGSIDITTTN SYEKEKLQER LAKLSGGVAV IRVGGASEVE VGEKKDRYDD
430 440 450 460 470 480
ALNATRAAVE EGILPGGGTA LVKASRVLDE VVVDNFDQKL GVDIIRKAIT RPAKQIIENA
490 500 510 520 530 540
GEEGSVIIGK LIDEYGDDFA KGYDASKSEY TDMLATGIID PFKVVRSGLV DASGVASLLA
550 560 570
TTEVAIVDAP EPPAAAGAGG MPGGMPGMPG MM
References and
documentation are available.
Number of amino acids: 572
Molecular weight: 60751.57
Theoretical pI: 5.23
Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 72
Atomic composition:
Carbon C 2659
Hydrogen H 4402
Nitrogen N 734
Oxygen O 849
Sulfur S 16
Formula: C
2659H
4402N
734O
849S
16
Total number of atoms: 8660
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10680
Abs 0.1% (=1 g/l) 0.176, assuming all pairs of Cys residues form cystines
Ext. coefficient 10430
Abs 0.1% (=1 g/l) 0.172, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.52
This classifies the protein as stable.
Aliphatic index: 96.68
Grand average of hydropathicity (GRAVY): -0.119