ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRSTISLLM NSAAQKTMTN SNFVLNAPKI TLTKVEQNIC NLLNDYTDLY NQKYHNKPEP
70 80 90 100 110 120
LTLRITGGWV RDKLLGQGSH DLDIAINVMS GEQFATGLNE YLQQHYAKYG AKPHNIHKID
130 140 150 160 170 180
KNPEKSKHLE TATTKLFGVE VDFVNLRSEK YTELSRIPKV CFGTPEEDAL RRDATLNALF
190 200 210 220 230 240
YNIHKGEVED FTKRGLQDLK DGVLRTPLPA KQTFLDDPLR VLRLIRFASR FNFTIDPEVM
250 260 270 280 290 300
AEMGDPQINV AFNSKISRER VGVEMEKILV GPTPLLALQL IQRAHLENVI FFWHNDSSVV
310 320 330 340 350 360
KFNEENCQDM DKINHVYNDN ILNSHLKSFI ELYPMFLEKL PILREKIGRS PGFQQNFILS
370 380 390 400 410 420
AILSPMANLQ IIGNPKKKIN NLVSVTESIV KEGLKLSKND AAVIAKTVDS ICSYEEILAK
430 440 450 460 470 480
FADRSQLKKS EIGIFLRNFN GEWETAHFAS LSDAFLKIPK LETKKIELLF QNYNEFYSYI
490 500 510 520 530 540
FDNNLNNCHE LKPIVDGKQM AKLLQMKPGP WLGKINNEAI RWQFDNPTGT DQELITHLKA
ILPKYL
References and
documentation are available.
Number of amino acids: 546
Molecular weight: 62484.99
Theoretical pI: 8.32
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 2818
Hydrogen H 4459
Nitrogen N 755
Oxygen O 814
Sulfur S 17
Formula: C
2818H
4459N
755O
814S
17
Total number of atoms: 8863
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 50100
Abs 0.1% (=1 g/l) 0.802, assuming all pairs of Cys residues form cystines
Ext. coefficient 49850
Abs 0.1% (=1 g/l) 0.798, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.42
This classifies the protein as unstable.
Aliphatic index: 95.02
Grand average of hydropathicity (GRAVY): -0.346