ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRSTVSKAS LKICRHFHRE SIPYDKTIKN LLLPKDTKVI FQGFTGKQGT FHASISQEYG
70 80 90 100 110 120
TNVVGGTNPK KAGQTHLGQP VFASVKDAIK ETGATASAIF VPPPIAAAAI KESIEAEIPL
130 140 150 160 170 180
AVCITEGIPQ HDMLYIAEML QTQDKTRLVG PNCPGIINPA TKVRIGIQPP KIFQAGKIGI
190 200 210 220 230 240
ISRSGTLTYE AVQQTTKTDL GQSLVIGMGG DAFPGTDFID ALKLFLEDET TEGIIMLGEI
250 260 270 280 290 300
GGKAEIEAAQ FLKEYNFSRS KPMPVASFIA GTVAGQMKGV RMGHSGAIVE GSGTDAESKK
310 320
QALRDVGVAV VESPGYLGQA LLDQFAKFK
References and
documentation are available.
Number of amino acids: 329
Molecular weight: 35032.45
Theoretical pI: 8.59
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1566
Hydrogen H 2524
Nitrogen N 418
Oxygen O 467
Sulfur S 11
Formula: C
1566H
2524N
418O
467S
11
Total number of atoms: 4986
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 9065
Abs 0.1% (=1 g/l) 0.259, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.255, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.67
This classifies the protein as stable.
Aliphatic index: 89.00
Grand average of hydropathicity (GRAVY): -0.045