ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRTKVFATT VARISGIRRY IPIRTINTVT KKNISKIEKL CEVLEVNPDG YKGKERIPTK
70 80 90 100 110 120
ELTKLLYTTS RNMLVRVPMT GDLSTGNTFE TRNETLQKLG EQLIHLEINK MLTITFTNFN
130 140 150 160 170 180
QFNIMNKNFN YIHNLDRARV VNMDSISWLI KNSLKINQLA HLRIPANLPK EMGLTSSSND
190 200 210 220
FQNLNDWKVI LSFIGYLKLL EIKNDNKKFI ESIIKTICIP LINYHLRKS
References and
documentation are available.
Number of amino acids: 229
Molecular weight: 26586.21
Theoretical pI: 9.94
Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1194
Hydrogen H 1957
Nitrogen N 329
Oxygen O 336
Sulfur S 9
Formula: C
1194H
1957N
329O
336S
9
Total number of atoms: 3825
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20065
Abs 0.1% (=1 g/l) 0.755, assuming all pairs of Cys residues form cystines
Ext. coefficient 19940
Abs 0.1% (=1 g/l) 0.750, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.72
This classifies the protein as stable.
Aliphatic index: 105.11
Grand average of hydropathicity (GRAVY): -0.259