ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRTSTLFTK RVQPSLFSRN ILRLQSTAAI PKTQKGVIFY ENKGNLHYKD IPVPEPKPNE
70 80 90 100 110 120
ILINVKYSGV CHTDLHAWHG DWPLPVKLPL VGGHEGAGVV VKLGSNVKGW KVGDLAGIKW
130 140 150 160 170 180
LNGSCMTCEF CESGHESNCP DADLSGYTHD GSFQQFATAD AIQAAKIQQG TDLAEVAPIL
190 200 210 220 230 240
CAGVTVYKAL KEADLKAGDW VAISGAAGGL GSLAVQYATA MGYRVLGIDA GEEKEKLFKK
250 260 270 280 290 300
LGGEVFIDFT KTKNMVSDIQ EATKGGPHGV INVSVSEAAI SLSTEYVRPC GTVVLVGLPA
310 320 330 340 350 360
NAYVKSEVFS HVVKSINIKG SYVGNRADTR EALDFFSRGL IKSPIKIVGL SELPKVYDLM
370
EKGKILGRYV VDTSK
References and
documentation are available.
Number of amino acids: 375
Molecular weight: 40327.43
Theoretical pI: 8.50
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1812
Hydrogen H 2879
Nitrogen N 483
Oxygen O 532
Sulfur S 12
Formula: C
1812H
2879N
483O
532S
12
Total number of atoms: 5718
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45755
Abs 0.1% (=1 g/l) 1.135, assuming all pairs of Cys residues form cystines
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.125, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.44
This classifies the protein as stable.
Aliphatic index: 93.31
Grand average of hydropathicity (GRAVY): -0.041