ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRVAGRRLF SVSQRSSTAT SFVLSRDHTL SDGGNSSSAS RSVPSADLSS FNSYHRSVIR 

        70         80         90        100        110        120 
GFASQVITQG NEIGFGSEVP ATVEAVKTPN SKIVYDDHNH ERYPPGDPSK RAFAYFVLSG 

       130        140        150        160        170        180 
GRFVYASVLR LLVLKLIVSM SASKDVLALA SLEVDLGSIE PGTTVTVKWR GKPVFIRRRT 

       190        200        210        220        230        240 
EDDIKLANSV DVGSLRDPQE DSVRVKNPEW LIVVGVCTHL GCIPLPNAGD YGGWFCPCHG 

       250        260        270 
SHYDISGRIR KGPAPYNLEV PTYSFLEENK LLIG 

References and documentation are available.

Number of amino acids: 274

Molecular weight: 29901.90

Theoretical pI: 8.86

Amino acid composition: 
Ala (A)  16	  5.8%
Arg (R)  20	  7.3%
Asn (N)  10	  3.6%
Asp (D)  15	  5.5%
Cys (C)   4	  1.5%
Gln (Q)   4	  1.5%
Glu (E)  12	  4.4%
Gly (G)  23	  8.4%
His (H)   7	  2.6%
Ile (I)  13	  4.7%
Leu (L)  24	  8.8%
Lys (K)  11	  4.0%
Met (M)   2	  0.7%
Phe (F)  11	  4.0%
Pro (P)  16	  5.8%
Ser (S)  34	 12.4%
Thr (T)  12	  4.4%
Trp (W)   3	  1.1%
Tyr (Y)   9	  3.3%
Val (V)  28	 10.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1329
Hydrogen    H	      2096
Nitrogen    N	       376
Oxygen      O	       398
Sulfur      S	         6

Formula: C1329H2096N376O398S6
Total number of atoms: 4205

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30160
Abs 0.1% (=1 g/l)   1.009, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   1.000, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.96
This classifies the protein as unstable.



Aliphatic index: 88.14

Grand average of hydropathicity (GRAVY): -0.157