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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRVFVIFSL FIAAINASGE FTVLNRPKAI SFKGNDALES HYVGDVLYAS MGNAVSGDTN

70 80 90 100 110 120
WNGLTINDPF NLAKGVILVH VQGIGHVTTA GNVKTYELTG SGTDASLNAL AAELEAANEP

130 140 150 160 170 180
VCDINFEQFD DGVQAWKSCF GDFEAPAAKP TKHLNPSLHT ADKQFLQEVG FINSAADHLA

190 200 210 220 230 240
EMAKPSNVLM LRVSVDGVAK AHGEKSVAVE EANKLLSAAI SRLLAASQKS SDSVLFVQTT

250 260 270 280 290 300
EKDVAASRAK RDTIAASTTN PYNLAVYYGS DYPVIFNIIL WFMVVFGLSL LAICYAIAAM

310 320
DPGRDSIIYR MTSTRIKKDN


References and documentation are available.

Number of amino acids: 320 Molecular weight: 34421.16 Theoretical pI: 5.50
Amino acid composition: 
Ala (A) 42 13.1% Arg (R) 9 2.8% Asn (N) 20 6.2% Asp (D) 19 5.9% Cys (C) 3 0.9% Gln (Q) 7 2.2% Glu (E) 14 4.4% Gly (G) 20 6.2% His (H) 7 2.2% Ile (I) 19 5.9% Leu (L) 28 8.8% Lys (K) 17 5.3% Met (M) 7 2.2% Phe (F) 16 5.0% Pro (P) 10 3.1% Ser (S) 26 8.1% Thr (T) 17 5.3% Trp (W) 3 0.9% Tyr (Y) 9 2.8% Val (V) 27 8.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 33 Total number of positively charged residues (Arg + Lys): 26 Atomic composition: Carbon C 1540 Hydrogen H 2414 Nitrogen N 408 Oxygen O 466 Sulfur S 10 Formula: C1540H2414N408O466S10 Total number of atoms: 4838 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 30035 Abs 0.1% (=1 g/l) 0.873, assuming all pairs of Cys residues form cystines Ext. coefficient 29910 Abs 0.1% (=1 g/l) 0.869, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 24.44 This classifies the protein as stable. Aliphatic index: 94.87 Grand average of hydropathicity (GRAVY): 0.113