ProtParam
User-provided sequence:
10 20 30 40 50 60
MLRVFVIFSL FIAAINASGE FTVLNRPKAI SFKGNDALES HYVGDVLYAS MGNAVSGDTN
70 80 90 100 110 120
WNGLTINDPF NLAKGVILVH VQGIGHVTTA GNVKTYELTG SGTDASLNAL AAELEAANEP
130 140 150 160 170 180
VCDINFEQFD DGVQAWKSCF GDFEAPAAKP TKHLNPSLHT ADKQFLQEVG FINSAADHLA
190 200 210 220 230 240
EMAKPSNVLM LRVSVDGVAK AHGEKSVAVE EANKLLSAAI SRLLAASQKS SDSVLFVQTT
250 260 270 280 290 300
EKDVAASRAK RDTIAASTTN PYNLAVYYGS DYPVIFNIIL WFMVVFGLSL LAICYAIAAM
310 320
DPGRDSIIYR MTSTRIKKDN
References and
documentation are available.
Number of amino acids: 320
Molecular weight: 34421.16
Theoretical pI: 5.50
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1540
Hydrogen H 2414
Nitrogen N 408
Oxygen O 466
Sulfur S 10
Formula: C
1540H
2414N
408O
466S
10
Total number of atoms: 4838
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30035
Abs 0.1% (=1 g/l) 0.873, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 0.869, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 24.44
This classifies the protein as stable.
Aliphatic index: 94.87
Grand average of hydropathicity (GRAVY): 0.113