ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRYHMQGFS GYGVQYSPFF DNRLAVAAGS NFGLVGNGKL FILEIDRSGR IVEVNSFLTQ 

        70         80         90        100        110        120 
DCLFDLAWNE SHENQVLVAQ GDGTLRLFDT TFKEFPIAIF KEHEREVFSC NWNLVNRQNF 

       130        140        150        160        170        180 
LSSSWDGSIK IWSPLRKQSL MTLTPRPLEI TKMVDPLNAI ILKKKSFTGI SKNRNCVYQA 

       190        200        210        220        230        240 
QFSPHDQNLV LSCSGNSYAS LFDIRLPSGK NQNNFLVHSG LEALTCDFNK YRPYVVATGG 

       250        260        270        280        290        300 
VDNAIRIWDI RMLNKNESAT IKRTVPGQLH NSSCINEIPN AHGLAIRKVT WSPHHSNILM 

       310        320        330        340        350        360 
SASYDMTCRI WRDLSNDGAK ETYKTNSTDA TKGSIFNFTQ HSEFVFGADW SLWGKPGYVA 

       370 
STAWDGNLFV WNGLG 

References and documentation are available.

Number of amino acids: 375

Molecular weight: 42322.94

Theoretical pI: 8.59

Amino acid composition: 
Ala (A)  21	  5.6%
Arg (R)  18	  4.8%
Asn (N)  31	  8.3%
Asp (D)  19	  5.1%
Cys (C)   7	  1.9%
Gln (Q)  13	  3.5%
Glu (E)  14	  3.7%
Gly (G)  27	  7.2%
His (H)  10	  2.7%
Ile (I)  22	  5.9%
Leu (L)  34	  9.1%
Lys (K)  19	  5.1%
Met (M)   7	  1.9%
Phe (F)  23	  6.1%
Pro (P)  13	  3.5%
Ser (S)  35	  9.3%
Thr (T)  20	  5.3%
Trp (W)  11	  2.9%
Tyr (Y)  10	  2.7%
Val (V)  21	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1899
Hydrogen    H	      2901
Nitrogen    N	       523
Oxygen      O	       551
Sulfur      S	        14

Formula: C1899H2901N523O551S14
Total number of atoms: 5888

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    75775
Abs 0.1% (=1 g/l)   1.790, assuming all pairs of Cys residues form cystines


Ext. coefficient    75400
Abs 0.1% (=1 g/l)   1.782, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.96
This classifies the protein as stable.



Aliphatic index: 80.08

Grand average of hydropathicity (GRAVY): -0.277