ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLRYYGATRN LPLVFSINKL MLRASSFTRP FHYSSYSLQN GDTPDKGSTN KNEIRTPNNA 

        70         80         90        100        110        120 
VWKENIELQW QHLKKKLNEL YSRFNFHRDQ LSFQVNKAKK SIQEANRKLS EQENEINDSR 

       130        140        150        160        170        180 
LNYNKDELTS AKIEGLPSER EQHRKKWSRK LEFYFDSLQE TLFTATRALN DVTGYSGIQK 

       190        200        210        220        230        240 
LKSSISLMEK KLEATKKEHK LFKAQYANAI DERAQSQREV NELLQRQSAW SSSDLERFTQ 

       250        260        270        280        290        300 
LYKNDALNAR QEQELKNKVK EIESKEEQLN DDLYRAILTR YHEEQIWSDK IRRTSTWGTF 

       310        320        330        340        350        360 
ILMGMNIFLF IVLQLLLEPW KRKRLVGSFE DKVKSALNEY AKEQNMKMDK LLPGKSSEVT 

       370        380        390        400        410        420 
DQGNTENSIV EEHIEQRGEC KINTAEIDRP EVATAETTTT EMKSFRDIWE RIKALFVTLK 

       430        440        450 
SIQYRKLDAP LVFDTLEFYL YSISLVSMTI LVSGLI 

References and documentation are available.

Number of amino acids: 456

Molecular weight: 53570.82

Theoretical pI: 9.01

Amino acid composition: 
Ala (A)  24	  5.3%
Arg (R)  29	  6.4%
Asn (N)  29	  6.4%
Asp (D)  20	  4.4%
Cys (C)   1	  0.2%
Gln (Q)  25	  5.5%
Glu (E)  45	  9.9%
Gly (G)  13	  2.9%
His (H)   7	  1.5%
Ile (I)  26	  5.7%
Leu (L)  51	 11.2%
Lys (K)  43	  9.4%
Met (M)   9	  2.0%
Phe (F)  19	  4.2%
Pro (P)   9	  2.0%
Ser (S)  39	  8.6%
Thr (T)  27	  5.9%
Trp (W)   8	  1.8%
Tyr (Y)  16	  3.5%
Val (V)  16	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      2381
Hydrogen    H	      3782
Nitrogen    N	       662
Oxygen      O	       723
Sulfur      S	        10

Formula: C2381H3782N662O723S10
Total number of atoms: 7558

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67840
Abs 0.1% (=1 g/l)   1.266, assuming all pairs of Cys residues form cystines


Ext. coefficient    67840
Abs 0.1% (=1 g/l)   1.266, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.85
This classifies the protein as unstable.



Aliphatic index: 81.29

Grand average of hydropathicity (GRAVY): -0.747