ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSAAEGILL SIATVEAGLG VLGNTFIALV NCMDWAKNNK LSMTGFLLIG LATSRIFIVW 

        70         80         90        100        110        120 
LLTLDAYAKL FYPSKYFSSS LIEIISYIWM TVNHLTVWFA TSLSIFYFLK IANFSDCVFL 

       130        140        150        160        170        180 
WLKRRTDKAF VFLLGCLLTS WVISFSFVVK VMKDGKVNHR NRTSEMYWEK RQFTINYVFL 

       190        200        210        220        230        240 
NIGVISLFMM TLTACFLLIM SLWRHSRQMQ SGVSGFRDLN TEAHVKAIKF LISFIILFVL 

       250        260        270        280        290        300 
YFIGVSIEII CIFIPENKLL FIFGFTTASI YPCCHSFILI LSNSQLKQAF VKVLQGLKFF 

References and documentation are available.

Number of amino acids: 300

Molecular weight: 34416.19

Theoretical pI: 9.46

Amino acid composition: 
Ala (A)  17	  5.7%
Arg (R)   9	  3.0%
Asn (N)  13	  4.3%
Asp (D)   6	  2.0%
Cys (C)   7	  2.3%
Gln (Q)   6	  2.0%
Glu (E)   8	  2.7%
Gly (G)  14	  4.7%
His (H)   5	  1.7%
Ile (I)  31	 10.3%
Leu (L)  41	 13.7%
Lys (K)  17	  5.7%
Met (M)  10	  3.3%
Phe (F)  31	 10.3%
Pro (P)   3	  1.0%
Ser (S)  27	  9.0%
Thr (T)  17	  5.7%
Trp (W)   8	  2.7%
Tyr (Y)   9	  3.0%
Val (V)  21	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1631
Hydrogen    H	      2509
Nitrogen    N	       381
Oxygen      O	       401
Sulfur      S	        17

Formula: C1631H2509N381O401S17
Total number of atoms: 4939

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57785
Abs 0.1% (=1 g/l)   1.679, assuming all pairs of Cys residues form cystines


Ext. coefficient    57410
Abs 0.1% (=1 g/l)   1.668, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.75
This classifies the protein as stable.



Aliphatic index: 119.57

Grand average of hydropathicity (GRAVY): 0.788