ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSAISKVST LKSCTRYLTK CNYHASAKLL AVKTFSMPAM SPTMEKGGIV SWKYKVGEPF 

        70         80         90        100        110        120 
SAGDVILEVE TDKSQIDVEA LDDGKLAKIL KDEGSKDVDV GEPIAYIADV DDDLATIKLP 

       130        140        150        160        170        180 
QEANTANAKS IEIKKPSADS TEATQQHLKK ATVTPIKTVD GSQANLEQTL LPSVSLLLAE 

       190        200        210        220        230        240 
NNISKQKALK EIAPSGSNGR LLKGDVLAYL GKIPQDSVNK VTEFIKKNER LDLSNIKPIQ 

       250        260        270        280        290        300 
LKPKIAEQAQ TKAADKPKIT PVEFEEQLVF HAPASIPFDK LSESLNSFMK EAYQFSHGTP 

       310        320        330        340        350        360 
LMDTNSKYFD PIFEDLVTLS PREPRFKFSY DLMQIPKANN MQDTYGQEDI FDLLTGSDAT 

       370        380        390        400        410 
ASSVRPVEKN LPEKNEYILA LNVSVNNKKF NDAEAKAKRF LDYVRELESF 

References and documentation are available.

Number of amino acids: 410

Molecular weight: 45361.75

Theoretical pI: 5.55

Amino acid composition: 
Ala (A)  36	  8.8%
Arg (R)   8	  2.0%
Asn (N)  20	  4.9%
Asp (D)  29	  7.1%
Cys (C)   2	  0.5%
Gln (Q)  16	  3.9%
Glu (E)  30	  7.3%
Gly (G)  15	  3.7%
His (H)   4	  1.0%
Ile (I)  24	  5.9%
Leu (L)  40	  9.8%
Lys (K)  45	 11.0%
Met (M)   8	  2.0%
Phe (F)  16	  3.9%
Pro (P)  23	  5.6%
Ser (S)  35	  8.5%
Thr (T)  23	  5.6%
Trp (W)   1	  0.2%
Tyr (Y)  11	  2.7%
Val (V)  24	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2022
Hydrogen    H	      3246
Nitrogen    N	       524
Oxygen      O	       634
Sulfur      S	        10

Formula: C2022H3246N524O634S10
Total number of atoms: 6436

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22015
Abs 0.1% (=1 g/l)   0.485, assuming all pairs of Cys residues form cystines


Ext. coefficient    21890
Abs 0.1% (=1 g/l)   0.483, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.58
This classifies the protein as stable.



Aliphatic index: 86.63

Grand average of hydropathicity (GRAVY): -0.410