ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSARCLKSI YFKRSFSQLG HIKPPKHIRN EPVKPFRNID LKDWDLLRAS LMKFKSSSLE 

        70         80         90        100        110        120 
VPLVINGERI YDNNERALFP QTNPANHQQV LANVTQATEK DVMNAVKAAK DAKKDWYNLP 

       130        140        150        160        170        180 
FYDRSAIFLK AADLISTKYR YDMLAATMLG QGKNVYQAEI DCITELSDFF RYYVKYASDL 

       190        200        210        220        230        240 
YAQQPVESAD GTWNKAEYRP LEGFVYAVSP FNFTAIAANL IGAPALMGNT VVWKPSQTAA 

       250        260        270        280        290        300 
LSNYLLMTVL EEAGLPKGVI NFIPGDPVQV TDQVLADKDF GALHFTGSTN VFKSLYGKIQ 

       310        320        330        340        350        360 
SGVVEGKYRD YPRIIGETGG KNFHLVHPSA NISHAVLSTI RGTFEFQGQK CSAASRLYLP 

       370        380        390        400        410        420 
ESKSEEFLSD MFGILQSQNV VPMNTSASPI SGGNLRGFMG PVIHEQSFDK LVKVIEDAKK 

       430        440        450        460        470        480 
DPELEILYGG QYDKSQGWFV GPTVIKAKRP DHPYMSTEFF GPILTVYEYP DTEFNEICDI 

       490        500        510        520        530        540 
IDNTSQYALT GAIFAKDRKA IEYADEKLKF SAGNFYINDK CTGAVVSQQW FGGARMSGTD 

       550        560        570 
DKAGGPNILS RFVSIRNTKE NFYELTDFKY PSNYE 

References and documentation are available.

Number of amino acids: 575

Molecular weight: 64435.20

Theoretical pI: 6.54

Amino acid composition: 
Ala (A)  47	  8.2%
Arg (R)  21	  3.7%
Asn (N)  31	  5.4%
Asp (D)  34	  5.9%
Cys (C)   5	  0.9%
Gln (Q)  23	  4.0%
Glu (E)  31	  5.4%
Gly (G)  39	  6.8%
His (H)   9	  1.6%
Ile (I)  34	  5.9%
Leu (L)  44	  7.7%
Lys (K)  42	  7.3%
Met (M)  12	  2.1%
Phe (F)  34	  5.9%
Pro (P)  30	  5.2%
Ser (S)  41	  7.1%
Thr (T)  28	  4.9%
Trp (W)   6	  1.0%
Tyr (Y)  29	  5.0%
Val (V)  35	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2917
Hydrogen    H	      4474
Nitrogen    N	       758
Oxygen      O	       858
Sulfur      S	        17

Formula: C2917H4474N758O858S17
Total number of atoms: 9024

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    76460
Abs 0.1% (=1 g/l)   1.187, assuming all pairs of Cys residues form cystines


Ext. coefficient    76210
Abs 0.1% (=1 g/l)   1.183, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.50
This classifies the protein as stable.



Aliphatic index: 78.73

Grand average of hydropathicity (GRAVY): -0.314