ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSDTIDTKQ QQQQLHVLFI DSYDSFTYNV VRLIEQQTDI SPGVNAVHVT TVHSDTFQSM 

        70         80         90        100        110        120 
DQLLPLLPLF DAIVVGPGPG NPNNGAQDMG IISELFENAN GKLDEVPILG ICLGFQAMCL 

       130        140        150        160        170        180 
AQGADVSELN TIKHGQVYEM HLNDAARACG LFSGYPDTFK STRYHSLHVN AEGIDTLLPL 

       190        200        210        220        230        240 
CTTEDENGIL LMSAQTKNKP WFGVQYHPES CCSELGGLLV SNFLKLSFIN NVKTGRWEKK 

       250        260        270        280        290        300 
KLNGEFSDIL SRLDRTIDRD PIYKVKEKYP KGEDTTYVKQ FEVSEDPKLT FEICNIIREE 

       310        320        330        340        350        360 
KFVMSSSVIS ENTGEWSIIA LPNSASQVFT HYGAMKKTTV HYWQDSEISY TLLKKCLDGQ 

       370        380        390        400        410        420 
DSDLPGSLEV IHEDKSQFWI TLGKFMENKI IDNHREIPFI GGLVGILGYE IGQYIACGRC 

       430        440        450        460        470        480 
NDDENSLVPD AKLVFINNSI VINHKQGKLY CISLDNTFPV ALEQSLRDSF VRKKNIKQSL 

       490        500        510        520        530        540 
SWPKYLPEEI DFIITMPDKL DYAKAFKKCQ DYMHKGDSYE MCLTTQTKVV PSAVIEPWRI 

       550        560        570        580        590        600 
FQTLVQRNPA PFSSFFEFKD IIPRQDETPP VLCFLSTSPE RFLKWDADTC ELRPIKGTVK 

       610        620        630        640        650        660 
KGPQMNLAKA TRILKTPKEF GENLMILDLI RNDLYELVPD VRVEEFMSVQ EYATVYQLVS 

       670        680        690        700        710        720 
VVKAHGLTSA SKKTRYSGID VLKHSLPPGS MTGAPKKITV QLLQDKIESK LNKHVNGGAR 

       730        740        750        760        770        780 
GVYSGVTGYW SVNSNGDWSV NIRCMYSYNG GTSWQLGAGG AITVLSTLDG ELEEMYNKLE 


SNLQIFM 

References and documentation are available.

Number of amino acids: 787

Molecular weight: 88544.14

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  32	  4.1%
Arg (R)  23	  2.9%
Asn (N)  40	  5.1%
Asp (D)  48	  6.1%
Cys (C)  16	  2.0%
Gln (Q)  36	  4.6%
Glu (E)  49	  6.2%
Gly (G)  53	  6.7%
His (H)  17	  2.2%
Ile (I)  54	  6.9%
Leu (L)  76	  9.7%
Lys (K)  56	  7.1%
Met (M)  19	  2.4%
Phe (F)  34	  4.3%
Pro (P)  37	  4.7%
Ser (S)  60	  7.6%
Thr (T)  46	  5.8%
Trp (W)  11	  1.4%
Tyr (Y)  28	  3.6%
Val (V)  52	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 97
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      3966
Hydrogen    H	      6197
Nitrogen    N	      1033
Oxygen      O	      1192
Sulfur      S	        35

Formula: C3966H6197N1033O1192S35
Total number of atoms: 12423

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   103220
Abs 0.1% (=1 g/l)   1.166, assuming all pairs of Cys residues form cystines


Ext. coefficient   102220
Abs 0.1% (=1 g/l)   1.154, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.07
This classifies the protein as unstable.



Aliphatic index: 87.65

Grand average of hydropathicity (GRAVY): -0.266