ProtParam
User-provided sequence:
10 20 30 40 50 60
MLSENTTILM ANGEIKDIAN VTANSYVMCA DGSAARVINV TQGYQKIYNI QQKTKHRAFE
70 80 90 100 110 120
GEPGRLDPRR RTVYQRLALQ CTAGHKLSVR VPTKPLLEKS GRNATKYKVR WRNLQQCQTL
130 140 150 160 170 180
DGRIIIIPKN HHKTFPMTVE GEFAAKRFIE EMERSKGEYF NFDIEVRDLD YLDAQLRISS
190 200 210 220 230 240
CIRFGPVLAG NGVLSKFLTG RSDLVTPAVK SMAWMLGLWL GDSTTKEPEI SVDSLDPKLM
250 260 270 280 290 300
ESLRENAKIW GLYLTVCDDH VPLRAKHVRL HYGDGPDENR KTRNLRKNNP FWKAVTILKF
310 320 330 340 350 360
KRDLDGEKQI PEFMYGEHIE VREAFLAGLI DSDGYVVKKG EGPESYKIAI QTVYSSIMDG
370 380 390 400 410 420
IVHISRSLGM SATVTTRSAR EEIIEGRKVQ CQFTYDCNVA GGTTSQNVLS YCRSGHKTRE
430 440 450 460 470 480
VPPIIKREPV YFSFTDDFQG ESTVYGLTIE GHKNFLLGNK IEVKSCRGCC VGEQLKISQK
490 500 510 520 530 540
KNLKHCVACP RKGIKYFYKD WSGKNRVCAR CYGRYKFSGH HCINCKYVPE AREVKKAKDK
550 560 570 580
GEKLGITPEG LPVKGPECIK CGGILQFDAV RGPHKSCGNN AGARIC
References and
documentation are available.
Number of amino acids: 586
Molecular weight: 66089.13
Theoretical pI: 9.32
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 93
Atomic composition:
Carbon C 2917
Hydrogen H 4659
Nitrogen N 841
Oxygen O 847
Sulfur S 32
Formula: C
2917H
4659N
841O
847S
32
Total number of atoms: 9296
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67030
Abs 0.1% (=1 g/l) 1.014, assuming all pairs of Cys residues form cystines
Ext. coefficient 65780
Abs 0.1% (=1 g/l) 0.995, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.28
This classifies the protein as unstable.
Aliphatic index: 78.00
Grand average of hydropathicity (GRAVY): -0.471