ProtParam
User-provided sequence:
10 20 30 40 50 60
MLSFAARRSL ITQKCAASTY SGLTSADFLK KKTNEPILEY RKGSKERQDL EAALAFYSKN
70 80 90 100 110 120
ITEVPLRIGD KKITNKLEQK QVIPSDHRST LAKYTYATGE QIQEAIDVAL QARGAWERKP
130 140 150 160 170 180
LKERAEILLR AADLAAGKYR MKLNASTMLG QGKNIIQAEI DAACELIDFF RFNAVFAMEL
190 200 210 220 230 240
EHYEPISTKI TKNTMQFRGM EGFVAAIAPF NFTAIGGNLP TAPALMGNVS LWKPSNTAVL
250 260 270 280 290 300
SNYIIYELLE EAGMPPGILS FLPSDGPVFG DVITASPHLS AVNFTGSVPT FKTIWRKVAE
310 320 330 340 350 360
NLDNYVTFPK LIGECGGKNF HFVHPSAHVD AVAAGTARSA WEYSGQKCSA CSRMYAPKSI
370 380 390
WPKILEKISA IHKEIKLGDF TGSRWIRLSV GCDR
References and
documentation are available.
Number of amino acids: 394
Molecular weight: 43528.18
Theoretical pI: 9.17
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 1959
Hydrogen H 3090
Nitrogen N 528
Oxygen O 563
Sulfur S 15
Formula: C
1959H
3090N
528O
563S
15
Total number of atoms: 6155
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 51255
Abs 0.1% (=1 g/l) 1.178, assuming all pairs of Cys residues form cystines
Ext. coefficient 50880
Abs 0.1% (=1 g/l) 1.169, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.65
This classifies the protein as stable.
Aliphatic index: 85.30
Grand average of hydropathicity (GRAVY): -0.159