Home  |  Contact

ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSFAARRSL ITQKCAASTY SGLTSADFLK KKTNEPILEY RKGSKERQDL EAALAFYSKN

70 80 90 100 110 120
ITEVPLRIGD KKITNKLEQK QVIPSDHRST LAKYTYATGE QIQEAIDVAL QARGAWERKP

130 140 150 160 170 180
LKERAEILLR AADLAAGKYR MKLNASTMLG QGKNIIQAEI DAACELIDFF RFNAVFAMEL

190 200 210 220 230 240
EHYEPISTKI TKNTMQFRGM EGFVAAIAPF NFTAIGGNLP TAPALMGNVS LWKPSNTAVL

250 260 270 280 290 300
SNYIIYELLE EAGMPPGILS FLPSDGPVFG DVITASPHLS AVNFTGSVPT FKTIWRKVAE

310 320 330 340 350 360
NLDNYVTFPK LIGECGGKNF HFVHPSAHVD AVAAGTARSA WEYSGQKCSA CSRMYAPKSI

370 380 390
WPKILEKISA IHKEIKLGDF TGSRWIRLSV GCDR


References and documentation are available.

Number of amino acids: 394 Molecular weight: 43528.18 Theoretical pI: 9.17
Amino acid composition: 
Ala (A) 45 11.4% Arg (R) 19 4.8% Asn (N) 16 4.1% Asp (D) 14 3.6% Cys (C) 6 1.5% Gln (Q) 11 2.8% Glu (E) 25 6.3% Gly (G) 26 6.6% His (H) 7 1.8% Ile (I) 29 7.4% Leu (L) 33 8.4% Lys (K) 30 7.6% Met (M) 9 2.3% Phe (F) 19 4.8% Pro (P) 19 4.8% Ser (S) 28 7.1% Thr (T) 23 5.8% Trp (W) 6 1.5% Tyr (Y) 12 3.0% Val (V) 17 4.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 39 Total number of positively charged residues (Arg + Lys): 49 Atomic composition: Carbon C 1959 Hydrogen H 3090 Nitrogen N 528 Oxygen O 563 Sulfur S 15 Formula: C1959H3090N528O563S15 Total number of atoms: 6155 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 51255 Abs 0.1% (=1 g/l) 1.178, assuming all pairs of Cys residues form cystines Ext. coefficient 50880 Abs 0.1% (=1 g/l) 1.169, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 36.65 This classifies the protein as stable. Aliphatic index: 85.30 Grand average of hydropathicity (GRAVY): -0.159