ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSIILLLMQ LVAADIAVVG PSLDQSFDAS GGTAKIPIQW LFTPNTPSQD DFTSLTFSLC 

        70         80         90        100        110        120 
SGPNYKIEAF KVIGKLSDIG TTDFEAEVSQ SVGANGYYYV QIYAATTDGY TIHYSPRFKL 

       130        140        150        160        170        180 
TGMTGSKLPD TLLITAPPTP ETRVTTGDLG ATIDSKSFDI PYGEQNGKAK FAPMQTQPGT 

       190        200        210        220        230        240 
KITATTWSRR YATSAVSFFT SLTATPVQHT TLTPGWSYYI SSDYNYAPPA PFPSDNGGWY 

       250        260 
DPKKRQSFTT RKLNMDKLRK RRQTS 

References and documentation are available.

Number of amino acids: 265

Molecular weight: 29046.77

Theoretical pI: 8.78

Amino acid composition: 
Ala (A)  20	  7.5%
Arg (R)   9	  3.4%
Asn (N)   7	  2.6%
Asp (D)  16	  6.0%
Cys (C)   1	  0.4%
Gln (Q)  12	  4.5%
Glu (E)   5	  1.9%
Gly (G)  19	  7.2%
His (H)   2	  0.8%
Ile (I)  15	  5.7%
Leu (L)  19	  7.2%
Lys (K)  15	  5.7%
Met (M)   5	  1.9%
Phe (F)  13	  4.9%
Pro (P)  20	  7.5%
Ser (S)  25	  9.4%
Thr (T)  34	 12.8%
Trp (W)   4	  1.5%
Tyr (Y)  14	  5.3%
Val (V)  10	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      1311
Hydrogen    H	      2014
Nitrogen    N	       334
Oxygen      O	       400
Sulfur      S	         6

Formula: C1311H2014N334O400S6
Total number of atoms: 4065

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42860
Abs 0.1% (=1 g/l)   1.476, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   1.476, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.69
This classifies the protein as stable.



Aliphatic index: 68.53

Grand average of hydropathicity (GRAVY): -0.319