ProtParam
User-provided sequence:
10 20 30 40 50 60
MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD KPKSILTDDM
70 80 90 100 110 120
LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK RQTSTDIKRE KYANWFYIFS
130 140 150 160 170 180
LSALTGTAIY MARDWEPQES EELKKDIDNG YTLSLMYKRF KARFNSMFTY FQEPPFPDLL
190 200 210 220 230 240
PPPPPPPYQR PLTLVITLED FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN
250 260 270 280 290 300
YMMYSDKIAE KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK
310 320 330 340 350 360
LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN LAEEFDHRVK
370 380 390 400 410 420
KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE EGQKNYLMFM KMIEEEKEKI
430 440 450 460 470
RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK IQLEKQKEVD ALFEEEKKKK KIAESK
References and
documentation are available.
Number of amino acids: 476
Molecular weight: 55098.71
Theoretical pI: 6.76
Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 72
Atomic composition:
Carbon C 2473
Hydrogen H 3864
Nitrogen N 652
Oxygen O 743
Sulfur S 15
Formula: C
2473H
3864N
652O
743S
15
Total number of atoms: 7747
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 64290
Abs 0.1% (=1 g/l) 1.167, assuming all pairs of Cys residues form cystines
Ext. coefficient 64290
Abs 0.1% (=1 g/l) 1.167, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.43
This classifies the protein as unstable.
Aliphatic index: 72.94
Grand average of hydropathicity (GRAVY): -0.747