ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSIRRHAKT VASSCTNLTQ KRTYVDVYMK WKRDPYFDNI EHILRSSQLK SVVSLKNCIV 

        70         80         90        100        110        120 
QEPNRCIPIS AISKKTRQFD VSTKIAHFLR KFPSIFEEFV GPEYNLPWFR LTPEATELDR 

       130        140        150        160        170        180 
QERVVYQTSA DDLRDRLKKL ILMSKDNVLP LSIVQGMKWY LGLPDDYLQF PDMNLDSSFR 

       190        200        210        220        230        240 
FVDMEDGVKG LAVDYNGGDK VLSVLQKNAM KKRRGEVSLE EIEFPLFPSK GCRLRVKIED 

       250        260        270        280        290        300 
WLMEFQKLPY VSPYDDYSCL DPSSDIAEKR VVGFLHELLC LFVEHSAERK KLLCLKKHFG 

       310        320        330        340        350        360 
LPQKVHKAFE RHPQIFYLSM KNKTCTAILR EPYRDKASVE THPVLGVRKK YIQLMKNSEL 

       370        380 
ILKSRRNSFG FRDEGVVDKD LDLDFEG 

References and documentation are available.

Number of amino acids: 387

Molecular weight: 45208.44

Theoretical pI: 9.09

Amino acid composition: 
Ala (A)  13	  3.4%
Arg (R)  27	  7.0%
Asn (N)  12	  3.1%
Asp (D)  28	  7.2%
Cys (C)   8	  2.1%
Gln (Q)  13	  3.4%
Glu (E)  25	  6.5%
Gly (G)  15	  3.9%
His (H)   9	  2.3%
Ile (I)  18	  4.7%
Leu (L)  44	 11.4%
Lys (K)  37	  9.6%
Met (M)  10	  2.6%
Phe (F)  21	  5.4%
Pro (P)  19	  4.9%
Ser (S)  29	  7.5%
Thr (T)  12	  3.1%
Trp (W)   4	  1.0%
Tyr (Y)  14	  3.6%
Val (V)  29	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2037
Hydrogen    H	      3224
Nitrogen    N	       552
Oxygen      O	       574
Sulfur      S	        18

Formula: C2037H3224N552O574S18
Total number of atoms: 6405

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43360
Abs 0.1% (=1 g/l)   0.959, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   0.948, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.68
This classifies the protein as unstable.



Aliphatic index: 87.57

Grand average of hydropathicity (GRAVY): -0.429