ProtParam
User-provided sequence:
10 20 30 40 50 60
MLSKRIVTAL NTAVKVQNAG IATTARGMAG ASGSEVSKIL EERILGTETG INLEETGKVL
70 80 90 100 110 120
SIGDGIARVY GLKNIQAEEM VEFDSGIKGM AMNLDVDNVG VVVFGNDKVI REGDIVKRTG
130 140 150 160 170 180
AIVDVPVGDG LLGRVVDALG NPIDGKGPIA NARRSRVEVK APGIIPRLSV REPMVTGVKA
190 200 210 220 230 240
VDSLVPIGRG QRELIIGDRQ TGKTAIAIDT IINQKRFNDA GDDKKKLFCI YVAVGQKRST
250 260 270 280 290 300
VAQIVKRLTD AGAMDYTIVV SATASDAAPL QFLAPYSGCA MGEHFRDNGK HALIIFDDLS
310 320 330 340 350 360
KQAVAYRQMS LLLRRPPGRE AYPGDVFYLH SRLLERAAKM NNSLGGGSLT ALPVIETQAG
370 380 390 400 410 420
DVSAYIPTNV ISITDGQIFL ETELFYKGVR PAINVGLSVS RVGSAAQTKA MKQVAGSMKL
430 440 450 460 470 480
ELAQYREVAA FAQFGSDLDA STQQLLNRGV RLTELLKQGQ YVPMGIEEQV GVIYAGVKGY
490 500 510 520 530
LDKVDPSAIT KFEKEFLAHL RSSQQALLKT IREEGQISPQ TDAQLKDVVV NFLATFKP
References and
documentation are available.
Number of amino acids: 538
Molecular weight: 57787.58
Theoretical pI: 8.98
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 2553
Hydrogen H 4187
Nitrogen N 719
Oxygen O 772
Sulfur S 15
Formula: C
2553H
4187N
719O
772S
15
Total number of atoms: 8246
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19495
Abs 0.1% (=1 g/l) 0.337, assuming all pairs of Cys residues form cystines
Ext. coefficient 19370
Abs 0.1% (=1 g/l) 0.335, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.51
This classifies the protein as stable.
Aliphatic index: 100.41
Grand average of hydropathicity (GRAVY): -0.026