ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSLRTPGFQ GESVQFSPLV ENKIAVAAVT HYGLGGSGRL YIHDVTPKGI QVCQHYDVED 

        70         80         90        100        110        120 
SLFGVRWSQN CENQVYACCG DGSLRLFDLT MPSKPIHKWK EHKAEIVAID TNTVDRRIVV 

       130        140        150        160        170        180 
TGSWDGTIKL WLGNLPNSVQ TLNNGSNSRI LTVATHYSSP NLLGYTSSDG LCKFWDFRSS 

       190        200        210        220        230        240 
DKFMSIEIPN QITCMNWSKS NHRMVYTADN NNLVYCYDIA NLKTPLSVLS GHQLAVRSIK 

       250        260        270        280        290        300 
SSNSAHDLLA TASYDMTSRI FDPEQHSCIR KVDLHSEFVR DVDWSDFGDG SWIASVGWDE 


SLYIWNAF 

References and documentation are available.

Number of amino acids: 308

Molecular weight: 34587.90

Theoretical pI: 5.97

Amino acid composition: 
Ala (A)  15	  4.9%
Arg (R)  13	  4.2%
Asn (N)  19	  6.2%
Asp (D)  22	  7.1%
Cys (C)   8	  2.6%
Gln (Q)  10	  3.2%
Glu (E)  10	  3.2%
Gly (G)  20	  6.5%
His (H)  11	  3.6%
Ile (I)  18	  5.8%
Leu (L)  26	  8.4%
Lys (K)  13	  4.2%
Met (M)   6	  1.9%
Phe (F)  11	  3.6%
Pro (P)  10	  3.2%
Ser (S)  35	 11.4%
Thr (T)  17	  5.5%
Trp (W)  10	  3.2%
Tyr (Y)  11	  3.6%
Val (V)  23	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1535
Hydrogen    H	      2347
Nitrogen    N	       421
Oxygen      O	       465
Sulfur      S	        14

Formula: C1535H2347N421O465S14
Total number of atoms: 4782

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    71890
Abs 0.1% (=1 g/l)   2.078, assuming all pairs of Cys residues form cystines


Ext. coefficient    71390
Abs 0.1% (=1 g/l)   2.064, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.81
This classifies the protein as stable.



Aliphatic index: 82.24

Grand average of hydropathicity (GRAVY): -0.258