ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSPERRPSM AERRPSFFSF TQNPSPLVVP HLAGIEDPLP ATTPDKVDVL IAGTGMVESV 

        70         80         90        100        110        120 
LAAALAWQGS NVLHIDKNDY YGDTSATLTV DQIKRWVNEV NEGSVSCYKN AKLYVSTLIG 

       130        140        150        160        170        180 
SGKYSSRDFG IDLSPKILFA KSDLLSILIK SRVHQYLEFQ SLSNFHTYEN DCFEKLTNTK 

       190        200        210        220        230        240 
QEIFTDQNLP LMTKRNLMKF IKFVLNWEAQ TEIWQPYAER TMSDFLGEKF KLEKPQVFEL 

       250        260        270        280        290        300 
IFSIGLCYDL NVKVPEALQR IRRYLTSFDV YGPFPALCSK YGGPGELSQG FCRSAAVGGA 

       310        320        330        340        350        360 
TYKLNEKLVS FNPTTKVATF QDGSKVEVSE KVIISPTQAP KDSKHVPQQQ YQVHRLTCIV 

       370        380        390        400        410        420 
ENPCTEWFNE GESAAMVVFP PGSLKSGNKE VVQAFILGAG SEICPEGTIV WYLSTTEQGP 

       430        440        450        460        470        480 
RAEMDIDAAL EAMEMALLRE SSSGLENDEE IVQLTGNGHT IVNSVKLGQS FKEYVPRERL 

       490        500        510        520        530        540 
QFLFKLYYTQ YTSTPPFGVV NSSFFDVNQD LEKKYIPGAS DNGVIYTTMP SAEISYDEVV 

       550        560        570        580        590        600 
TAAKVLYEKI VGSDDDFFDL DFEDEDEIQA SGVANAEQFE NAIDDDDDVN MEGSGEFVGE 


MEI 

References and documentation are available.

Number of amino acids: 603

Molecular weight: 67373.96

Theoretical pI: 4.64

Amino acid composition: 
Ala (A)  37	  6.1%
Arg (R)  18	  3.0%
Asn (N)  27	  4.5%
Asp (D)  36	  6.0%
Cys (C)   8	  1.3%
Gln (Q)  27	  4.5%
Glu (E)  51	  8.5%
Gly (G)  38	  6.3%
His (H)   7	  1.2%
Ile (I)  31	  5.1%
Leu (L)  50	  8.3%
Lys (K)  35	  5.8%
Met (M)  13	  2.2%
Phe (F)  34	  5.6%
Pro (P)  31	  5.1%
Ser (S)  50	  8.3%
Thr (T)  34	  5.6%
Trp (W)   6	  1.0%
Tyr (Y)  23	  3.8%
Val (V)  47	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 87
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      3019
Hydrogen    H	      4650
Nitrogen    N	       766
Oxygen      O	       939
Sulfur      S	        21

Formula: C3019H4650N766O939S21
Total number of atoms: 9395

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67770
Abs 0.1% (=1 g/l)   1.006, assuming all pairs of Cys residues form cystines


Ext. coefficient    67270
Abs 0.1% (=1 g/l)   0.998, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.30
This classifies the protein as stable.



Aliphatic index: 81.13

Grand average of hydropathicity (GRAVY): -0.272