ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSRAIFRNP VINRTLLRAR PGAYHATRLT KNTFIQSRKY SDAHDEETFE EFTARYEKEF 

        70         80         90        100        110        120 
DEAYDLFEVQ RVLNNCFSYD LVPAPAVIEK ALRAARRVND LPTAIRVFEA LKYKVENEDQ 

       130        140 
YKAYLDELKD VRQELGVPLK EELFPSSS 

References and documentation are available.

Number of amino acids: 148

Molecular weight: 17341.61

Theoretical pI: 5.80

Amino acid composition: 
Ala (A)  15	 10.1%
Arg (R)  14	  9.5%
Asn (N)   7	  4.7%
Asp (D)   9	  6.1%
Cys (C)   1	  0.7%
Gln (Q)   4	  2.7%
Glu (E)  16	 10.8%
Gly (G)   2	  1.4%
His (H)   2	  1.4%
Ile (I)   5	  3.4%
Leu (L)  15	 10.1%
Lys (K)   9	  6.1%
Met (M)   1	  0.7%
Phe (F)   9	  6.1%
Pro (P)   7	  4.7%
Ser (S)   7	  4.7%
Thr (T)   7	  4.7%
Trp (W)   0	  0.0%
Tyr (Y)   8	  5.4%
Val (V)  10	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	       778
Hydrogen    H	      1214
Nitrogen    N	       214
Oxygen      O	       232
Sulfur      S	         2

Formula: C778H1214N214O232S2
Total number of atoms: 2440

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.687, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.687, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.51
This classifies the protein as unstable.



Aliphatic index: 82.43

Grand average of hydropathicity (GRAVY): -0.576