ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSRIVSNNA TRSVMCHQAQ VGILYKTNPV RTYATLKEVE MRLKSIKNIE KITKTMKIVA 

        70         80         90        100        110        120 
STRLSKAEKA KISAKKMDEA EQLFYKNAET KNLDVEATET GAPKELIVAI TSDKGLCGSI 

       130        140        150        160        170        180 
HSQLAKAVRR HLNDQPNADI VTIGDKIKMQ LLRTHPNNIK LSINGIGKDA PTFQESALIA 

       190        200        210        220        230        240 
DKLLSVMKAG TYPKISIFYN DPVSSLSFEP SEKPIFNAKT IEQSPSFGKF EIDTDANVPR 

       250        260        270        280        290        300 
DLFEYTLANQ MLTAMAQGYA AEISARRNAM DNASKNAGDM INRYSILYNR TRQAVITNEL 

       310 
VDIITGASSL G 

References and documentation are available.

Number of amino acids: 311

Molecular weight: 34350.52

Theoretical pI: 9.34

Amino acid composition: 
Ala (A)  32	 10.3%
Arg (R)  14	  4.5%
Asn (N)  21	  6.8%
Asp (D)  15	  4.8%
Cys (C)   2	  0.6%
Gln (Q)  11	  3.5%
Glu (E)  18	  5.8%
Gly (G)  13	  4.2%
His (H)   4	  1.3%
Ile (I)  28	  9.0%
Leu (L)  24	  7.7%
Lys (K)  28	  9.0%
Met (M)  11	  3.5%
Phe (F)   8	  2.6%
Pro (P)  11	  3.5%
Ser (S)  25	  8.0%
Thr (T)  22	  7.1%
Trp (W)   0	  0.0%
Tyr (Y)   9	  2.9%
Val (V)  15	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1506
Hydrogen    H	      2473
Nitrogen    N	       421
Oxygen      O	       466
Sulfur      S	        13

Formula: C1506H2473N421O466S13
Total number of atoms: 4879

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13535
Abs 0.1% (=1 g/l)   0.394, assuming all pairs of Cys residues form cystines


Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.390, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.54
This classifies the protein as stable.



Aliphatic index: 89.49

Grand average of hydropathicity (GRAVY): -0.310