ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY ILCQFPYPSG 

        70         80         90        100        110        120 
ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA ENAAIERSIN PAIWTRDNIA 

       130        140        150        160        170        180 
KMKQQMQSML ANFDWDREIT TCDPEYYKFT QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL 

       190        200        210        220        230        240 
ANEQVDAQGR SWRSGAIVEK KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG 

       250        260        270        280        290        300 
ESVGAELVFK VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK 

       310        320        330        340        350        360 
SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC PGHDNRDFEF 

       370        380        390        400        410        420 
WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS TDGVLTKECG EHSGVLTVVA 

       430        440        450        460        470        480 
RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ RYWGTPIPII HCDNCGPVPV PESDLPVKLP 

       490        500        510        520        530        540 
ELEGLDTKGN PLSTIDEFVN VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF 

       550        560        570        580        590        600 
DREIASKNMP VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV 

       610        620        630        640        650        660 
QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS KYNGADPNEC 

       670        680        690        700        710        720 
ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV LHLTKNILSL EKDLAISKDY 

       730        740        750        760        770        780 
KTPTDLNDAE VKFHNDFQRF LKSITESFEV NLSLNTVISD YMKLTNILES ALKKGEVRNE 

       790        800        810        820        830        840 
MIVQNLQKLV TVIYPAVPSI SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR 

       850        860        870        880        890 
VKFMYTADKN FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK 

References and documentation are available.

Number of amino acids: 894

Molecular weight: 101920.31

Theoretical pI: 8.57

Amino acid composition: 
Ala (A)  49	  5.5%
Arg (R)  35	  3.9%
Asn (N)  47	  5.3%
Asp (D)  51	  5.7%
Cys (C)  12	  1.3%
Gln (Q)  34	  3.8%
Glu (E)  56	  6.3%
Gly (G)  47	  5.3%
His (H)  16	  1.8%
Ile (I)  60	  6.7%
Leu (L)  71	  7.9%
Lys (K)  80	  8.9%
Met (M)  24	  2.7%
Phe (F)  46	  5.1%
Pro (P)  54	  6.0%
Ser (S)  59	  6.6%
Thr (T)  46	  5.1%
Trp (W)  19	  2.1%
Tyr (Y)  27	  3.0%
Val (V)  61	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 107
Total number of positively charged residues (Arg + Lys): 115

Atomic composition:

Carbon      C	      4613
Hydrogen    H	      7189
Nitrogen    N	      1211
Oxygen      O	      1322
Sulfur      S	        36

Formula: C4613H7189N1211O1322S36
Total number of atoms: 14371

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   145480
Abs 0.1% (=1 g/l)   1.427, assuming all pairs of Cys residues form cystines


Ext. coefficient   144730
Abs 0.1% (=1 g/l)   1.420, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.75
This classifies the protein as stable.



Aliphatic index: 82.42

Grand average of hydropathicity (GRAVY): -0.366