ProtParam
User-provided sequence:
10 20 30 40 50 60
MLSRVAKRAF SSTVANPYKV TVLGAGGGIG QPLSLLLKLN HKVTDLRLYD LKGAKGVATD
70 80 90 100 110 120
LSHIPTNSVV KGFTPEEPDG LNNALKDTDM VLIPAGVPRK PGMTRDDLFA INASIVRDLA
130 140 150 160 170 180
AATAESAPNA AILVISNPVN STVPIVAQVL KNKGVYNPKK LFGVTTLDSI RAARFISEVE
190 200 210 220 230 240
NTDPTQERVN VIGGHSGITI IPLISQTNHK LMSDDKRHEL IHRIQFGGDE VVKAKNGAGS
250 260 270 280 290 300
ATLSMAHAGA KFANAVLSGF KGERDVIEPS FVDSPLFKSE GIEFFASPVT LGPDGIEKIH
310 320 330
PIGELSSEEE EMLQKCKETL KKNIEKGVNF VASK
References and
documentation are available.
Number of amino acids: 334
Molecular weight: 35649.96
Theoretical pI: 8.46
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1584
Hydrogen H 2579
Nitrogen N 437
Oxygen O 480
Sulfur S 7
Formula: C
1584H
2579N
437O
480S
7
Total number of atoms: 5087
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.125, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.125, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.06
This classifies the protein as stable.
Aliphatic index: 96.05
Grand average of hydropathicity (GRAVY): -0.101