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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSRVAKRAF SSTVANPYKV TVLGAGGGIG QPLSLLLKLN HKVTDLRLYD LKGAKGVATD

70 80 90 100 110 120
LSHIPTNSVV KGFTPEEPDG LNNALKDTDM VLIPAGVPRK PGMTRDDLFA INASIVRDLA

130 140 150 160 170 180
AATAESAPNA AILVISNPVN STVPIVAQVL KNKGVYNPKK LFGVTTLDSI RAARFISEVE

190 200 210 220 230 240
NTDPTQERVN VIGGHSGITI IPLISQTNHK LMSDDKRHEL IHRIQFGGDE VVKAKNGAGS

250 260 270 280 290 300
ATLSMAHAGA KFANAVLSGF KGERDVIEPS FVDSPLFKSE GIEFFASPVT LGPDGIEKIH

310 320 330
PIGELSSEEE EMLQKCKETL KKNIEKGVNF VASK


References and documentation are available.

Number of amino acids: 334 Molecular weight: 35649.96 Theoretical pI: 8.46
Amino acid composition: 
Ala (A) 30 9.0% Arg (R) 12 3.6% Asn (N) 18 5.4% Asp (D) 17 5.1% Cys (C) 1 0.3% Gln (Q) 6 1.8% Glu (E) 20 6.0% Gly (G) 28 8.4% His (H) 8 2.4% Ile (I) 23 6.9% Leu (L) 30 9.0% Lys (K) 27 8.1% Met (M) 6 1.8% Phe (F) 13 3.9% Pro (P) 19 5.7% Ser (S) 25 7.5% Thr (T) 19 5.7% Trp (W) 0 0.0% Tyr (Y) 3 0.9% Val (V) 29 8.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 37 Total number of positively charged residues (Arg + Lys): 39 Atomic composition: Carbon C 1584 Hydrogen H 2579 Nitrogen N 437 Oxygen O 480 Sulfur S 7 Formula: C1584H2579N437O480S7 Total number of atoms: 5087 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 4470 Abs 0.1% (=1 g/l) 0.125, assuming all pairs of Cys residues form cystines Ext. coefficient 4470 Abs 0.1% (=1 g/l) 0.125, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 33.06 This classifies the protein as stable. Aliphatic index: 96.05 Grand average of hydropathicity (GRAVY): -0.101