ProtParam
User-provided sequence:
10 20 30 40 50 60
MLSSIKPSSS SFSTAISGSV RRSIPTKLKF SPLLIIKNCH NQSFNANVVS HQKPLHISSA
70 80 90 100 110 120
SNFKREVKVE AYEADRSRPL DINIELPDEQ SAQKLKIGIY FATWWALNVV FNIYNKKVLN
130 140 150 160 170 180
AFPYPWLTST LSLACGSLMM LVSWATRIAD APKTDLEFWK TLFPVAVAHT IGHVAATVSM
190 200 210 220 230 240
SKVAVSFTHI IKSGEPAFSV LVSRFFMGET FPLPVYLSLL PIIGGCALAA ITELNFNITG
250 260 270 280 290 300
FMGAMISNLA FVFRNIFSKK GMKGKSVSGM NYYACLSMMS LVILTPFSIA VEVPQMWAAG
310 320 330 340 350 360
WQNAVSQVGP NFVWWVVAQS VFYHLYNQVS YMSLDQISPL TFSIGNTMKR ISVIVASIII
370 380
FHTPIQPVNA LGAAIAIFGT FLYSQAKQ
References and
documentation are available.
Number of amino acids: 388
Molecular weight: 42796.16
Theoretical pI: 9.64
Total number of negatively charged residues (Asp + Glu): 16
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1977
Hydrogen H 3066
Nitrogen N 494
Oxygen O 529
Sulfur S 18
Formula: C
1977H
3066N
494O
529S
18
Total number of atoms: 6084
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66140
Abs 0.1% (=1 g/l) 1.545, assuming all pairs of Cys residues form cystines
Ext. coefficient 65890
Abs 0.1% (=1 g/l) 1.540, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.30
This classifies the protein as unstable.
Aliphatic index: 100.28
Grand average of hydropathicity (GRAVY): 0.403