ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSSKTFLNL VRTSRCRMSS SFAPKPAERT AQHSASIWVE FTTLAAETKA VNLGQGFPDS 

        70         80         90        100        110        120 
PAPKFVTDLL ENLSKHPELT AAHQYTRGYG HPMLVDILAK MYSHFYNVQV DPMNEVLVTV 

       130        140        150        160        170        180 
GAYLSLYYAF LGWVNKGDEV LIIEPAYDCY YPQVKFAGGV PVPVVMNLAE GATSASQFTI 

       190        200        210        220        230        240 
DFADMESKIN EKTKMLVINN PHNPTGKLFS RHELEKLAEI AKKHNLIVIA DEVYEFHVWD 

       250        260        270        280        290        300 
KNDMVRFASL PGMYERTISI GSAGKAFSVT GWKLGWAVGP KQLLEPLKAI HQNCVFTCST 

       310        320        330        340        350        360 
PTQMAIAEAF RLDWPKFLSD PENSYLATGL SGELRAKRDK LAKMLEEGNF RPIIPDAGYF 

       370        380        390        400        410        420 
MLADYVHLKE GINLPTEEDP DDFVFSRWLC REKKLAVIPP SAFYSARDNK LKNSNMVRLC 

       430        440 
YFKKDETLDA AEEILKKLGK Q 

References and documentation are available.

Number of amino acids: 441

Molecular weight: 49617.13

Theoretical pI: 6.61

Amino acid composition: 
Ala (A)  40	  9.1%
Arg (R)  16	  3.6%
Asn (N)  20	  4.5%
Asp (D)  22	  5.0%
Cys (C)   6	  1.4%
Gln (Q)  10	  2.3%
Glu (E)  30	  6.8%
Gly (G)  23	  5.2%
His (H)  11	  2.5%
Ile (I)  19	  4.3%
Leu (L)  44	 10.0%
Lys (K)  34	  7.7%
Met (M)  14	  3.2%
Phe (F)  23	  5.2%
Pro (P)  26	  5.9%
Ser (S)  28	  6.3%
Thr (T)  22	  5.0%
Trp (W)   7	  1.6%
Tyr (Y)  17	  3.9%
Val (V)  29	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2250
Hydrogen    H	      3484
Nitrogen    N	       582
Oxygen      O	       643
Sulfur      S	        20

Formula: C2250H3484N582O643S20
Total number of atoms: 6979

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    64205
Abs 0.1% (=1 g/l)   1.294, assuming all pairs of Cys residues form cystines


Ext. coefficient    63830
Abs 0.1% (=1 g/l)   1.286, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.81
This classifies the protein as stable.



Aliphatic index: 83.85

Grand average of hydropathicity (GRAVY): -0.207